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1TISSUE OPTICAL CLEARING FOR 3D PROFILING OF WHOLE ORGANS
TINGTING YU1
1 Britton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China [email protected]
ABSTRACT
Acquiring the three-dimensional (3D) structure of biological tissues is essential for research in life sciences. Modern optical imaging techniques and fluorescent labeling technologies have provided vital tools for obtaining high-resolution information on the 3D structures of biological tissues. However, the turbid nature of biological tissues limits the depth of light penetration, leading to restricted applications for large tissues or whole organs. Tissue optical clearing technology takes a different approach by making the tissues transparent using various physical and chemical strategies to reduce the attenuation of light in tissues, and providing a new approach for the 3D imaging of whole tissue organs. Here, we will introduce our progress in the research of tissue optical clearing methods, namely SOLID, a method minimizing tissue distortion for brain-wide profiling of diverse architectures, as well as the improved blood vessel labeling techniques.
REFERENCES
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