CC BY
42
Protistology ■ 37
2 - Shirshov'Institute ofOceanology, Russian Academy
of Sciences, Russia
The results of the species composition, abundance and biomass of heterotrophic nanoflagellates (HNF) were obtained for the first time in the Laptev Sea in August-September 2014-2015. An attempt was made to assess the role of HNF in grazing productions of bacterioplankton (PB) and virioplankton (PV). 24 species and forms of HNF 9 taxa and groups of uncertain systematic position were found. Kinetoplastea Honigberg, 1963 were characterized by the highest species diversity. The HNF community dominated by bacteriophages (eighteen species), also found four omnivorous and two carnivorous species. Nhnf ranged from 108-651 cells/ml, the biomass — 0.3-3.5 mg C/m3. NHNF was three orders of magnitude lower than NB, and BHNF was 1.6-63.3% (mean 24.4+4.0%) biomass of bacterioplankton. Between NHNF and NB were detected high positive correlation (R=0.63, p=0.05). The virus particles (diameter of 200-531 nm) potentially can be edible objects for HNF. However, the ratio of the number of large viruses at NHNF was low - 42-417. HNF could consume a significant amount of virus attached to the walls and inside bacteria. The number of bacteria with attached viruses was 8.7-26.5% NB. On bacteria it was up to 11 virus particles. The number ofbacteriophage-viruses in infected cells reached 111 particle/cell. Guided by literature data on clearance rates of water by Arctic HNF (Sherr et al., 1997), calculated that in the water column of the Laptev sea the HNF community consumed 1.1-30.4% (average 8.3%) daily PB and 1.3-77.2% (average 14.9%) the daily PV. The research supported by the RFBR grant 14-04-00130.
THE STATUS OF THE SPECIES BALANTI-DIUM ELONGATUM FROM THE GUT OF EUROPEAN COMMON FROG Kornilova O.A.1, Chistyakova L.V.2, Kostygov A. Yu.34
1 - Herzen State Pedagogical University of Russia, St. Petersburg
2 - Centre of Core Facility of SPSU "Culturing collections of microorganisms", St. Petersburg, Russia
3 - Zoological Institute of the Russian Academy of Sciences
4 - Life Science Research Centre, Faculty of Science, University of Ostrava
The representatives of the genus Balantidium Clapare de et Lachmann, 1858 have several common morphological features: sacciform or
slightly elongated body completely covered with cilia forming dense longitudinal rows. In the anterior part of the body, there is an opening of vestibulum. Such features are clearly visible under light micro-scope. Because balantidia have very few other morphological characters suitable for taxonomy, their species identity is usually determined using morphometric data. Comparative analysis of several described Balantidium spp. indicated that many of these specific names might be synonyms. All balantidia inhabiting the intestine of mammals and birds were recognized to belong to a single species — Balantidium coli, recently moved to the genus Balantioides Alexeieff, 1931. We supposed that some species from amphibians may be synonyms (balantidia are polymorphic) and some of them may belong to different genera. For solving this issue, we used modern electron microscopic techniques and molecular phylogenetic analysis. We studied the morphology of Balantidium entozoon and B. elongatum from the gut of European common frog Rana temporaria Linnaeus, 1758 using light and electron microscopy. The distinguishing features of B. elongatum are the long thin fusiform body and relatively short vestibulum. However, other traits (number of kinetes and the distance between them) are indistinguishable between two species. Their intracellular structures also showed no differences. The sequences of 18S rRNA gene of both species were identical to each other and to that of B. entozoon from the GenBank. Thus B. elongatum must be considered as a younger synonym of B. entozoon.
BRIDGING THE GAP BETWEEN TRADITIONAL TAXONOMY AND TRANSCRIP-TOMICS IN ARCELLINIDS (AMOEBOZOA) Kosakyan Anush1, Brown Matthew W.2 3, Lara Enrique4, Mitchell Edward A.D.45, Lahr Daniel J.G.1
1 - Laboratory of Evolutionary Protistology, Institute of Biosciences, University of Sao Paulo, Matao, Travessa 14,Cidade Universitaria, 05508-090 - Sao Paulo, SP, Brasil
2 - Department ofBiological Sciences, Mississippi State University, Mississippi State, 39762, Mississippi, USA
3 - Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, 39762, Mississippi, USA
4 - Laboratory ofSoilBiology, University ofNeuchatel, Rue Emile-Argand 1, 2000 Neuchatel, Switzerland
5 - Jardin Botanique de Neuchatel, Chemin du Perthuis-du-Sault 58, 2000Neuchatel, Switzerland. [email protected]
