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16 • "PROTIST—2016
Basic Research (grant nos. 14-04-00500, 14-0400554,15-29-02518).
THE CHLAMYDOPHRYIDAE REVISITED WITH SPECIAL FOCUS ON THE GENUS LECYTHIUM
Dumack Kenneth1, Baumann Christina1, Muller Marina2, Bonkowski Michael1
1 - University of Cologne
2 - Leibniz-Centre for Agricultural Landscape Research ZALF
[email protected] Although testate amoebae have attracted interest of protistologists for more than 150 years, some groups especially those with a hyaline test are still poorly known. One of those fairly understudied groups is the family Chlamydophryidae. They are difficult to culture and therefore only old, sometimes obscure, species descriptions are available. Further, no molecular data was obtained and therefore in modern mass sequencing studies they are only recognized as OTUs of unknown affinity. Nevertheless, they are widely distributed, can occur in high abundances in freshwater and terrestrial habitats, show interesting feeding behavior and have a high diversity in morphology. This talk will give an overview of the up to date knowledge on the Chlamydophryidae and will further focus on one of its genera, Lecythium that was first described by Hertwig & Lesser in 1874. We (a) established 7 cultures of 5 Lecythium species and provide detailed morphological as well as ecological observations (b) obtained SSU sequences and conducted phylo-genetic analyses of the Tectofilosida, showing that Lecythium is closely related to the Pseudodifflugiidae and splits into a terrestrial and freshwater clade and (c) performed food choice experiments that underline the mycophagous and algivorous feeding behavior of Lecythium. Our talk will show that the Chlamydophryidae are a group of protists that are highly fascinating.
THE BASE OF CERCOMONAD RADIATION IS STILL IN FOR A SURPRISE, KRAKEN GEN. NOV.
Dumack Kenneth1, Schuster Julia1, Bass David2, Bonkowski Michael1
1 - University of Cologne
2 - Department of Life Sciences, Natural History Museum London, Cromwell Road, London [email protected]
The term 'filose amoebae' describes a highly poly-phyletic assemblage of protists whose phylogenetic placement can be unpredictable based on gross
morphology alone. We isolated 6 filose amoebae from soils of two European countries and describe a new genus of naked filose amoebae, Kraken gen. nov. comprising one new species Kraken carinae sp. nov. We provide a morphological description based on light microscopy and small subunit rRNA gene sequences (SSU rDNA). In culture, Kraken carinae strains were very slow-moving and preyed on bacteria using a network of filopodia. Phylogenetic analyses of SSU sequences reveal that Kraken are core Cercozoa, branching weakly at the base of the cercomonad radiation, most closely related to Paracercomonas, Metabolomonas, and Brevimastigomonas. Kraken sequences are >99% similar to an environmental sequence obtained from a freshwater lake in Antarctica, indicating that Kraken carinae is not exclusively soil dwelling, but also inhabits freshwater habitats.
FIRST RECORD OF BROMELIOPHRYA BRA-SILIENSIS FOISSNER, 2003 (CILIOPHORA: GLAUCOMIDAE) FROM TANK BROMELIADS IN MEXICO WITH SOME NOTES ON ITS DISTRIBUTION AND ECOLOGY Durán-Ramírez C. A.1, Mayén-Estrada R.1, Ciros-Pérez J.2
1 - Laboratorio de Protozoología, Departamento de Biología Comparada, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria s/n, Coyoacán, 04510Mexico City
2 - Proyecto de Investigación en Limnología Tropical. UIICSE, FESIztacala UNAM. Av. de los Barrios No. 1, Los Reyes Iztacala, Tlalnepantla 54090, Estado de México
[email protected] Bromeliophrya brasiliensis is a glaucomid bacteri-vorous ciliate described from terrestrial tank bromeliads in the Atlantic Coast of Brazil, and recorded in Paraná, Brazil and Ecuador from rupicolous bromeliads. The reduction of somatic ciliature and the presence of two kinetofragments, which migrate to the left margin of the buccal cavity, are the main cytological characteristics as compared with B. quadristicha which presents four kinetofragments and it has been recorded in the Antilles. The objective ofthis work is to describe the ciliate diversity from tank bromeliads in Mexico. We sampled 30 individuals of epiphytic tank bromeliads including five species of genera Catopsis and Tillandsia in three different temperate forest sites from Central and Southern Mexico, during the year 2015. The sites were located from 1336 to 2426 m a.s.l. Observation and identification of B. brasiliensis was performed using bright and DIC microcopy,
