CC BY
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90 • "PROTIST—2016
of pelagic diatom, Skeletonema marinoi JK029 (NC_028615, 38,515 bp) isolated from an interdidal site ofthe Sihwaho (37°18'38.91"N 126°36'16.35"E). Complete mtDNA of each species shows typical bacillariophycean mitochondrial contents, including of35-42 coding sequences (CDS), 2 ribosomal RNA (rRNA), and 25-26 transfer RNA (tRNA) genes. All CDS ofmtDNA aligned with all available diatoms data, including Phaeodactylum tricornutum (NC_016739), Fragilaria acus (NC_013710), and Thalassiosira pseudonana (NC_007405). Based on the saturation test and preliminary sequencing, we suggest mitochondrial markers for diatoms survey on the west coast of Korea.
HORIZONTAL TRANSFER OF A NOVEL SIX-GENE OPERON FROM A BACTERIUM INTO THE PLASTID GENOME OF EUSTIG-MATOPHYTE ALGAE
Yurchenko T.12, Sevcikova T.1, Strnad H.3, Butenko A.1, EliaS M.12
1 - University ofOstrava, Faculty ofScience, Department of Biology and Ecology, Life Science Research Centre, Ostrava, Czech Republic
2 - University ofOstrava, Faculty ofScience, Institute of Environmental Technologies, Ostrava, Czech Republic
3 - Institute ofMolecular Genetics, Czech Academy of Sciences, Prague, Czech Republic [email protected]
Genes in plastid genomes (plastomes) have been primarily inherited from the cyanobacterial plastid ancestor, but cases of genes acquired by horizontal gene transfer (HGT) have been occassionally reported from plastomes of different algal lineages. Here we report an interesting case of HGT-mediated gene acquisition revealed by sequencing plastomes of the eustigmatophyte algae Monodopsis sp. MarTras21 and Vischeria sp. CAUP 202. While the gene complement of the newly and previously sequenced eustigmatophyte plastomes proved to be highly conserved, those of Monodopsis sp. and Vischeria sp. harbour a cluster of six genes not reported from any plastid genome sequenced so far. All six genes have homologs in various bacteria, where they are usually organized in the same six-gene cluster, i.e. a putative operon. Phylogenetic analyses showed that the cluster from eustigmatophyte plastomes is nested among sequences from the order Cytophagales (phylum Bacteroidetes), with the cluster from Sporocytophaga myxococcoides constituting a robustly resolved sister group to the eustigmatophyte clade. Sequence analyses using different homology-detection tools failed to detect functionally characterized homologs of the protein
encoded by the first gene of the operon, whereas the remaining five proteins could be assigned only to broader enzyme superfamilies. Nevertheless, based on these analyses we speculate that the newly detected operon encodes enzymes of a pathway synthesizing a prenylated aromatic compound, possibly an antimicrobial or other protective substance. To our knowledge, this is the first report of an expansion ofthe metabolic capacity of a plastid mediated by HGT into the plastome.
SPECIES DIVERSITY AND MORPHOLOGY
OF CENTROHELID HELIOZOANS OF SOME
SITES IN EUROPEAN PART OF RUSSIA AND
SOUTH-EAST UKRAINE
Zagumyonnyi D.G.
Voronezh State University, Russia
The species composition and cytoskeleton morphology of centrohelid heliozoans from different water biotopes ofEuropean Russia and South-East Ukraine have been investigated. 21 species from 6 genera and 3 families have been found. Two forms (Acanthocystis sp. nov. 1. and Acanthocystis sp. nov. 2.) have appeared to be as new species. Following species Acanthocystis turfacea, Heterophrys myriopoda, Polyplacocystis symmetrica, Polyplacocystis coerulea, Acanthocystis dentate have been marked for SouthEast Ukraine for the first time. Also Acanthocystis dentata, Raineriophrys fortesca, Raineriophrys kilianii, Pterocystis foliaea and Pterocystis pulchra have been found for the Central Russia for the first time. Pterocystis pulchra has been revealed for heliozoan fauna of Russia firstly at all. Heterophrys marina has been found in fresh waters of Russia. Polyplacocystis symmetrica has been found in slightly salted biotopes. The morphological descriptions of all studied species have been given. New data on spicule structures of Acanthocystis dentate have been done. Most frequent species was Acanthocystis pectinata. This study was supported by the Russian Scientific Foundation (grant nos. 14-14-00515).
RUBISCO IN NON-PHOTOSYNTHETIC ALGA EUGLENA LONGA: DIVERGENT FEATURES, TRANSCRIPTOMIC ANALYSIS AND REGULATION OF COMPLEX FORMATION Zahonova K.1, Fussy Z.2, Obornik M.234, Elias M.1, Yurchenko V.12
1 - Life Science Research Centre, Department of Biology and Ecology and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 701 00 Ostrava, Czech Republic
2 - Institute of Parasitology, Biology Centre ASCR, 370 05 Ceske Budejovice, Czech Republic
