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1Impact Factor: SJIF 2023 - 5.95 2024 - 5.99
БИОЛОГИЧЕСКИЕ НАУКИ BIOLOGICAL SCIENCES
SILVER NANOPARTICLE FORMATION PROPERTIES OF LACTOBACILLUS
SPP. BACTERIAL STRAIN DI-10
JAFAROV M.M*., ASLANOVA A.C., ALAKBARLi S.N., ALKiSHiYEVA K.S.,
EYVAZOVA Q.i.
Baku State University, Azerbaijan AR Ministry of Science and Education, Institute of Microbiology , Azerbaijan
Summary. As a result of our research work, it was determined that Lactobacillus spp. the lactic acid bacteria strain DI-10 was recorded to produce silver nanoparticles by both color change (transparent to dark brown) and absorption at 410 nm wavelength in UV-VIS spectrophotometer.
Key words: strain, lactic acid bacteria, silver nanoparticles, UV-VIS spectrophotometr
Applied in many spheres, nanotechnology is developing day by day and is widely used in medicine and industry. The strong development of nanotechnology is based on the high potential of optical, chemical, electronic, photoelectrochemical properties of nanoparticles. First of all, physical and chemical methods were used to obtain various metal nanoparticles. At this time, the fact that they have an ecologically destructive effect creates a basis for the use of biosynthetic methods as an alternative. Recently, biological synthesis methods (plants, fungi and bacteria) have been widely used to obtain silver nanoparticles. In their work, many researchers have noted that, silver nanoparticles are synthesized by fungi and bacteria [1 - 5].
In this regard, the aim of our presented work was to study the properties of the formation of silver nanoparticles by Lactobacillus spp. lactic acid bacteria strain DI-10.
Isolated Lactobacillus spp. lactic acid bacteria strain DI-10 was isolated from the spontaneous sour milk product used in Dilagarda settlement of Fizuli district and we obtained its pure culture. The optimal nutrient medium, meat-peptone broth, was used to grow the microbial culture. After preparing meat-peptone broth, 5 flasks of 250 ml were taken and 200 ml of liquid nutrient medium was added to each flask. Nutrient media were placed in an autoclave and sterilized for 30 minutes at a temperature of 121°C at a pressure of 1 atmosphere and Lactobacillus spp., the biomass of DI-10 lactic acid bacteria strain was dissolved in 10 ml of sterile water and added to the flasks in equal amounts. The flasks were mixed and placed in a thermostat at 30°C for incubation. After 5 days of incubation, the formed microbial biomass was separated by centrifugation and 10 g of biomass was washed 3 times with 100 ml of distilled water. The washed biomass was added to a flask containing 100 ml of distilled water. Then, 1 ml of AgNO3 salt was mixed into the mixture, and both the experimental variant and the control (control) flask were placed in a thermostat at 30°C for incubation. From the 3rd day of incubation, a color change began to appear in the experimental variant. On the 7th day of the experiment, 10 ml of the mixture was filtered and analyzed in a UV-VIS spectrophotometer, the absorption spectrum at a wavelength of 410 nm was observed and the peak was recorded.
Thus, as a result of our research work, it was determined that Lactobacillus spp. the lactic acid bacteria strain DI-10 was recorded to produce silver nanoparticles by both color change (transparent to dark brown) and absorption at 410 nm wavelength in UV-VIS spectrophotometer.
Separation of this useful strain for the human body from the sour milk product and obtained silver nanoparticles from this strain is suitable for use in the field of medicine and industry in the future.
Impact Factor: SJIF 2023 - 5.95 2024 - 5.99
БИОЛОГИЧЕСКИЕ НАУКИ BIOLOGICAL SCIENCES
REFERENCES
1. Chaudhari, P. R., Masurkar, S. A., Shidore, V. B. & Kamble, S. P. Antimicrobial activity of extracellularly synthesized silver nanoparticles using Lactobacillus species obtained from VIZYLAC capsule. J. Appl. Pharm. Sci. 2(3), 25-29 (2012).
2. Dakhil, A. S. Biosynthesis of silver nanoparticle (AgNPs) using Lactobacillus and their effects on oxidative stress biomarkers in rats. J. King Saud Univ. Sci. 29(4), 462-467 (2017).
3. Ganbarov Kh.G, Jafarov M.M, Huseynova S.I, Muhammad Shoaib Alkishiyeva K.S Effect of environmental factors on the production of silver nanoparticles by SACCHAROMYCES ELLIPSOIDEUS BSU-XR1 strain// Biosciences Biotechnology Research Asia, 2023, Vol. 20, № 3, p. 837 - 844
4. Garmasheva, I. et al. Lactobacillus species mediated synthesis of silver nanoparticles and their antibacterial activity against opportunistic pathogens in vitro. Biolmpacts 6(4), 219-223 (2016).
5. Jafarov M.M., Гусейнова С. И., Азадалиева С. Ф. Особенности создания наночастиц серебра штаммами дрожжевого гриба Saccharomyces// Современная наука: актуальные проблемы теории и практики. Серия «Естественные и технические науки», 2022 , №11 - 2, c.18 - 21
