CC BY
100PHYSIOLOGICAL ACTIVITY OF PLANT GROWTH STIMULATORS
Khodanitska O.,
PhD of Agricultural Sciences, Associate Professor, Vinnytsia Mykhailo Kotsiubynskyi State Pedagogical University
Shevchuk O.,
PhD of Biological Sciences, Associate Professor, Vinnytsia Mykhailo Kotsiubynskyi State Pedagogical University
Tkachuk O.,
PhD of Biological Sciences, Associate Professor, Vinnytsia Mykhailo Kotsiubynskyi State Pedagogical University
Matviichuk O.
PhD of Biological Sciences, Associate Professor, Vinnytsia Mykhailo Kotsiubynskyi State Pedagogical University
Abstract
The article considers the physiological activity of plant growth stimulants based on phytohormones. Plant growth regulators by their nature are analogs of phytohormones or modifiers of their action. Therefore, the physiological effect of growth stimulants is determined by changes in the balance of biologically active substances in the plant organism. The use of developmental stimulants containing compounds of auxin, gibberellin, cytokinin and brassine nature is substantiated.Exogenous treatment of compounds with analogues of phytohormones, their antagonists or regulators of activity, intensively effect on the metabolic processes and lead to changes in growth processes, allows improving of the seed productivity of plants.
Keywords: phytohormones, plant growth stimulants, auxins, cytokinins, gibberellins, brassinosteroids, productivity, germination.
Regulation of plants growth and development is one of the important problems of modern crop production and plant physiology [1]. Today we know about 5,000 biologically active substances, some of which have found practical application in agricultural production. Growth regulators are increasingly used in modern technologies for crop production. They can be natural and synthetic organic compounds that affect metabolic processes in plants and stimulate or inhibit their growth and morphogenesis [2, 6].
Phytohormonal regulation of plant morphogenesis is a perspective direction in the development of agricultural technologies. Physiological activity in a plant organism is determined not by the content of particular groups of phytohormones, but mainly determined by the balance of biologically active substances [16].
Exogenous treatment of compounds with analogues of phytohormones, their antagonists or regulators of activity, intensively effect on the metabolic processes and lead to changes in growth processes, allows improving of the seed productivity of plants [3].
Growth regulators are substances of synthetic and natural origin that have biological activity and in microdoses can cause changes in morphophysiological processes and affect crop productivity. Growth regulators make it possible to regulate the growth and development of plants; more effectively realize the potential of varieties and hybrids that inherent in the genome by nature, selection or genetic engineering [7].
Plant growth regulators, depending on the direction of their influence on the plant organism, are divided into stimulators and inhibitors of plant growth and development [4]. The biologically active substances contained in such preparations cause a wide
range of protective reactions, increase of metabolic processes, resistance of plants to environmental stressors and improvement of product quality.
The action of plant growth stimulants of synthetic origin is associated with the acceleration of the processes of cell division, stretching and differentiation, increase of the linear size of plants, increase of the assimilation surface area and productivity of plants [5, 15].
Synthetic plant growth regulators are analogs of natural growth substances - phytohormones [8]. They accelerate the growth, development and productivity of plants due to increased activity or their content in tissues [11]. Modern growth stimulators are combined drugs that influence on the complex of phytohormones. Phytohormones, having a multifunctional action, regulate many biochemical processes in plants [9, 12]. Receptor proteins contained in the middle of the cell bind to hormones. The hormone-receptor complex is responsible for the regulation of physiological and biochemical processes in the plant.
There are two types of receptors: intracellular soluble receptor proteins that bind phytohormones and migrate between the cytoplasm and the nucleus; membrane receptor proteins that bind phytohormones from the extracellular space.
Intracellular receptor proteins, when they bind the phytohormone, cause gene expression and affect metabolism in the cell. Bind proteins of the first type are found for gibberellins, auxins and cytokinins. Membrane receptor proteins realize the physiological and biochemical role of phytohormone, increase the concentration of mediator metabolites in the cell. Accordingly, phytohormones activate special genes responsible for the synthesis of essential enzymes. Phytohormones determine the structure and function of ribosomes, cell
membranes, endoplasmic reticulum, changes cell metabolism. The genome of plants controls the biosynthesis of phytohormones themselves.
Plant growth stimulants of phytohormonal origin are classified into [1]:
1. Gibberellin drugs and analogues created on their basis (activol, gibrelat, gibreskol).
2. Auxin preparations and their synthetic analogues (heteroauxin, 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthylacetic acid, its potassium salt).
3. Cytokinin drugs and their synthetic analogues (kinetin, cytodef, 6-benzylaminopurine, diphenylurea), benzimidazole and benzimidazolone derivatives.
4. Brassinosteroids as stimulants of the immune system of plants (epibrasinolide, homobrasinolide, zircon).
5. Ethylene inhibitors (aviglycin and its hydrochloride, aminooxyacetic acid).
Synthetic analogues of phytohormones, in contrast to natural biologically active substances, are more stable in the plant organism and are characterized by a prolonged action over time. However, there are no regulators of plant growth of the universal type that would affect the development of plants at all stages of ontogenesis [10, 14].
It is noted that application of growth-regulating compounds increase in simultaneity and seed germination energy, accelerate the shoot and root system growth, which leads to improve the absorption of nutrients from the soil, the resistance of plant organism to the action of extreme environmental factors.
Phytohormones and their synthetic derivatives compounds stimulate to increase in the proliferation and accelerate the processes of cell differentiation, which caused formation of a more powerful root system and changes of leaf mesostructural organization. The enhanced development of assimilative apparatus leads to the intensification of organic substances synthesis, which are used for the needs of formation and generative organs loading, that is an important key to improving crop yields.
Growth stimulants are used to increase field germination of seeds, stimulate root formation and photosynthesis, accelerate ripening, increase yields, improve product quality, reduce disease and contain of heavy metals in products. Thus, an artificial analogue of auxins - p-indolylbutyric acid (0.25-0.5% aqueous-alcoholic solution) is used for rapid rooting of cuttings and the formation of the root system of plants [1].
Auxins play an important role in the vegetative reproduction of plants that are difficult to root, or to restore the root system when transplanting large plants due to their ability to accelerate root growth. In addition, they are used to obtain parthenocarpic fruits, thinning of flowers and ovaries in fruit trees, which are associated with the formation of ethylene, which in turn accelerates fruit ripening. Auxins are often used to kill dicotyledonous weeds (chlorophenoxy acids).
Cytokinins and their analogues play an important role in the regulation of plant growth and development. 6-benzylaminopurine (6-BAP) is used to increase plant resistance to disease and other stressors, as well as in the cultivation of tissues and organs [11].
The practical use of synthetic analogues of cyto-kinins is based on physiological properties and is associated with obtaining bushy forms of plants (removal of apical dominance), inhibition of leaf aging, induction of flowering and shifting the expression of plant sex with the formation of more female flowers.
Cytokinins with auxins are one of the components of nutrient environment in biotechnology and are necessary to accelerate organogenesis during the cultivation of plants in tissue culture. With the help of cyto-kinins and their analogues, it is possible to influence the formation of organs and flowering of plants under conditions of unfavorable photoperiodic regime. Cytokinin drugs are used to increase the resistance of plants to deficit of moisture, high and low temperatures, fungal pathogens.
Gibberellins are important for plant development, especially for stem growth. Low levels of gibberellins do not allow plants to reach natural heights. Therefore, inhibitors of gibberellin synthesis are widely used in agriculture to form stems artificially short: shorter and thicker stems provide better support, as well as better resistance to weather conditions.
Gibberellins are especially effective when the seeds come out of dormancy, to accelerate germination, increase germination energy. Seeds that germinate difficult are often soaked in solutions of gibberellic acid, after which the general germination improves.
It is known from the literature that gibberellins affect cell division. Gibberellins increase the linear size of the plant, stimulate cell stretching and division. A classic example of the use of gibberellin is the cultivation of grapes without seeds Treatment of plants with gibberellins can increase resistance to phytopathogens.
High physiological activity is characteristic of brassinosteroids, such as epine. Epine is a plant growth regulator, the active ingredient of which is a natural, environmentally friendly brassinosteroid, which even in microdoses has a high biological activity. It changes the phytohormonal balance in plants, can increase crop yields in stressful situations, resistance to disease, reduces the negative impact of plant uptake of radionu-clides and heavy metals.
Thus, plant growth and development stimulators are heterogeneous group of compounds used in agriculture to optimize productivity, improve harvesting conditions, store products and ensure its high quality characteristics.
References
1. Gritsayenko Z.M., Ponomarenko S.P., Karpenko V.P., Leontyuk I.B. 2008. Biologically active substances in crop production. 352.
2. Khodanitska, O.O., Kuryata, V.G., Shevchuk, O.A. et al. 2019. Effect of treptolem on morphogenesis and productivity of linseed plants. Ukrainian Journal of Ecology. 9 (2). 119-126.
3. Khodanitska, O.O., Shevchuk, O.A., Tkachuk, O.O., Shevchuk, V.V. 2019. Features of the anatomical structure of the autonomic organs and flax oil yield (Li-
num usitatissimum L.) at applications growth stimulants. Science Rise: Biological Science. 4 (20). 35-40. DOI:10.15587/2519-8025.2019.188317 8.
4. Khodanitska O.O. 2020. The influence of growth regulators on the oil content in the flax seeds Materialy XVI Mi^dzynarodowej naukowi-praktycznej konferencji , «Naukowa przestrzen Europy - 2020» , Volume 8 Przemysl: Nauka i studia. 3-5.
5. Khodanitska O.O., Dovgalenko T., Graboviy R. 2019. Application of growth regulators for flax crop. Materials of the XV International scientific and practical Conference Cutting-edge science - 2019 , April 30 - May 7, 2019. 7-9
6. Kolisnyk, O. M., Khodanitska, O. O., Bu-tenko, A. O., Lebedieva, N. A., Yakovets, L. A., Tkachenko, O. M., ... & Kurinnyi, O. V. 2020. Influence of foliar feeding on the grain productivity of corn hybrids in the conditions of the right-bank forest-steppe of Ukraine. Ukrainian Journal of Ecology, 10(2), 4044.
7. Kuryata V.G., Polyvanyi S.V., Rogach T. I., Khodanitska O. O., Rogach V. V. 2019. Influence of chlormequat chloride on morphogenesis, formation of donor-acceptor system and production process of oil crops. The Potential of Modern Science, 1. 130-156.
8. Kuryata, V.G., Golunova, L.A., Poprotska, I.V., Khodanitska, O.O. 2019. Symbiotic nitrogen fixation of soybean-rhizobium complexes and productivity of soybean culture as affected by the retardant chlormequat chloride.Ukrainian Journal of Ecology. 9 (2). 5-13.
9. Kuryata, V.G., Khodanitska, O.O. 2018. Features of anatomical structure, formation and functioning of leaf apparatus and productivity of linseed under chlormequatchloride treatment. Ukrainian Journal of Ecology. 8 (1). 918-926. https://doi.org/10.15421/2018_294
10. Kuryata, V.G., Polyvanyi, S.V., Shevchuk,
0.A. et al. 2019. Morphogenesis and the effectiveness of the production process of oil poppy under the complex action of retardant chlormequat chloride and growth stimulant treptolem. Ukrainian Journal of Ecology. 9 (1). 127-134.
11. Morgun V.V., Yavorskaya V.K., Dragovoz
1.V. 2002. The problem of growth regulators in the world and its solution in Ukraine. Physiology and biochemistry of cultural plants. 34, № 5. 371-376.
12. Polyvanyi, S.V., Golunova, L.A., Baiurko, N.V., Khodanitska, O.O., Shevchuk, V.V., Rogach, T.I., Tkachuk, O.O., Knyazyuk, O.V., Zavalnyuk, O. L., Shevchuk O.A. 2020. Morphogenesis of mustard white under the action of the antigibberellic preparation chlormequat chloride. Modern Phytomorphology. 14. 101-103.
13. Poprotska I., Kuryata V., Khodanitska O., Polyvanyi S., Golunova L., & Prysedsky Y. 2019. Effect of gibberellin and retardants on the germination of seeds with different types of reserve substances under the conditions of skoto-and photomorphogenesis. Bi-ologija, 65(4), 296-307.
14. Shevchuk, O.A., Kravets, O.O., Shevchuk, V.V., Khodanitska, O.O., Tkachuk, O.O. et al. 2020. Features of leaf mesostructure organization under plant growth regulators treatment on broad bean plants. Modern Phytomorphology. 14. 104-106. https://doi.org/10.5281/zenodo.200119
15. Shevchuk, O.A., Tkachuk, O.O., Kuryata, V.G., Khodanitska, O.O., Polyvanyi, S.V. 2019. Features of leaf photosynthetic apparatus of sugar beet under retardants treatment. Ukrainian Journal of Ecology. 9 (1). 115-120.
16. Yashchuk V.V., Dulnev P.G. 2012. Phytohor-mones in vegetable growing. Protection and quarantine plants. 58. 288-292.
