CC BY
3DOI 10.24412/CL-37135-2023-1-50-50
PHOTOSAFE NON-INVASIVE DETECTION OF DEEP-SEATED LESIONS VIA TRANSMISSION RAMAN SPECTROSCOPY
JIAN YE
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
ABSTRACT
Non-invasive localization of deep lesions remains a long-standing pursuit for clinical applications, and its key point lies in the detection and depth estimation of a single lesion in heterogeneous tissues. At present, full optical modalities are widely applied for biomedical sensing, diagnosis, and intraoperative guidance. However, due to the strong photon absorption and scattering of biological tissues, it is challenging to realize in vivo deep detections, particularly for those using the safe laser irradiance below biological maximum permissible exposure (MPE) [1]. We reported in vivo surface-enhanced transmission Raman spectroscopy (SETRS) to achieve the non-invasive and photosafe localization of deep lesion deeply hidden in either ex vivo thick tissues or in vivo mice model [2-4]. We synthesized the near-infrared SERS nanotags with single-nanoparticle detection sensitivity, and developed a home-built TRS system with an enlarged beam size to lower the laser power density to 0.264 W/cm2 , below the MPE criteria. By using the TRS system, we successfully demonstrated the detection of SERS nanotags through up to 14-cm-thick ex vivo porcine tissues, as well as in vivo imaging of "phantom" lesions labeled by SERS nanotags in an unshaved mouse under MPE. Furthermore, we theoretically and experimentally demonstrate a universal method to achieve the depth estimation of phantom lesions ex vivo tissues, and also realized in vivo accurate localization of deep sentinel lymph nodes in a live rat model. This work highlights the potential of transmission Raman-guided identification and noninvasive imaging toward clinically photosafe cancer diagnoses.
Figure: Schematic of non-invasive in vivo imaging of deep-seated tumor through living mice by using a transmission setup and ultrabright SERS contrast agent
REFERENCES
[1] Li Lin, Haoqi He, Ruiyang Xue, Yumin Zhang, Ziwen Wang, Shuming Nie, Jian Ye, Direct and quantitative assessments of near-infrared light attenuation and spectroscopic detection depth in biological tissues using surface-enhanced Raman scattering, Med-X,2023, 1(1), 9.
[2] Yumin Zhang, Ruoyu Chen, Fugang Liu, Peng Miao, Li Lin, and Jian Ye, In Vivo Surface-Enhanced Transmission Raman Spectroscopy under Maximum Permissible Exposure: Toward Photosafe Detection of Deep-Seated Tumors, Small Methods 2023, 7(2), 2201334.
[3] Yumin Zhang, Li Lin, Jian Ye. A rapid and universal method for depth estimation of lesions in heterogeneous tissues via photosafe ratiometric transmission Raman spectroscopy. VIEW, 2023,4(4), 20230022.
[4] Zongyu Wu, Binge Deng, Yutong Zhou, Haoqiang Xie, Yumin Zhang, Li Lin, and Jian Ye. Non-Invasive Detection, Precise Localization, and Perioperative Navigation of In Vivo Deep Lesions Using Transmission Raman Spectroscopy, Advanced Science, 2023, 2301721
