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12B-O-2
Increasing the thermal effect efficiency of NIR laser radiation on biological tissue using Yb-containing dielectric nanoparticles
S.A. Khrushchalina, A.N. Belyaev, O.S. Bushukina, P.A. Ryabochkina, I.A. Yurlov
National Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk 430005, Russia
Near and middle infrared (IR) lasers are widely used in dermatology [1-6]. Optical fibers are usually used for radiation delivery to tissues, which makes it possible to realize contact and non-contact treatment modes [1-8]. The thermal effect on a biotissue for contact treatment mode can be enhanced by the "blackening" of the fiber endface contacting directly with the tissue [4]. In this case, higher temperatures can be achieved in the contact zone (between the fiber endface and the biological tissue), and, as a consequence, the treatment efficiency can be increased [5]. In this paper, we propose another way for enhancing the thermal effect of NIR laser radiation on the biotissue surface for non-contact treatment mode, which consists in preliminary deposition of Yb-containing nanosized dielectric particles on it. The heating of these nanoparticles upon 980 nm excitation to the Yb3+ ions absorption band has been studied and described previously [7].
White adult 180-220 g wistar rats were used as experimental animals. The rat was fixed in the prone position and a 6x4 cm section was shaved on its back. A stand with rat was fixed on a motorized translation stage (Zaber Technologies Inc.), which ensured its precise movement. The selected regions of bioobjects were covered with a Yb-contained crystalline powders. A semiconductor laser diode with wavelength of 980 nm and maximal output power of 1 W was used as radiation source for in-vivo experiments. The histological examination of samples was carried out using a method described in [8]. All in-vivo experiments were performed at room temperature and in accordance with the provisions of the European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (Strasbourg, 1986), in compliance with the Directive 2010/63/EU and Declaration of Helsinki.
We have demonstrated that preliminary coating of a biological tissue with Yb-containing nanoparticles provides more pronounced its thermal damage under the 980 nm laser exposure than without coating. The damage degree of the tissue depends on the laser radiation power and the exposure time. Experiments in-vivo also allowed to evaluate the dynamics of tissue healing.
This work is financially supported by a grant from the President of the Russian Federation (MK-5500.2021.1.2).
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