CC BY
8
Bulletin of the International Scientific Surgical Association
Vol.3, N. 1, 200S
© A.K.Martusevich, A.S.Luzan, A.V.Bochkareva, 2008
A.K.Martusevich, A.S.Luzan, A.V.Bochkareva
FREE AND INITIATED CRYSTALLOGENESIS OF HUMAN BIOLOGICAL FLUIDS UNDER RECTAL OZONOTHERAPY
Nizhny Novgorod Institute of Traumatology and Orthopedics, Russia
Abstract:
Based on the saliva and intestinal colon cleaning waters teziocrystalloscopic and spectrometric analysis of the 12 healthy people and 12 patients after rectal ozone detoxication, which was accomplished by colon irrigation with the ozone-contained isotonic sodium chloride solution we found out peculiarities of the free biosubstratum crystals building. Biosubstance crystallogenesis characteristics were evaluated after one procedure and the whole rectal ozonother-apy course. We disposed that one rectal ozonotherapy and the whole course changed free crystallogenesis of the saliva and intestinal colon cleaning waters in different ways. Key words :
saliva, excrements, crystallogenesis, spectroscopy
Biosub-strata
Saliva
Healthy people
Excrements
At present time indicatory role of the crystallo-graphic methods are actively studied. They allow monitoring patients condition by biological fluids crystallization characteristics [1-3, 5]. As some authors think use of the crystallographic methods is very informative in human pathology diagnostics, management effectiveness estimation and pathogenesis investigation [1-5]. The importance of crystal building and initiation properties of biosubstrata include metabolic changes verification possibilities [3, 5]. In connection with it the above-mentioned methods could be applied to control systematic ozonotherapy effectiveness. Today this problem is insufficiently studied, because the informative methods of patients condition dynamics estimation according treatment are absent.
A lot of human biological substrata are studied (blood serum, saliva, urine, gastric juice, tears etc.). Tendencies for use some of biological substances (first of all blood serum) as a universal substrata for crystalloscopic analysis are wide spread [1, 5]. But maximal metabolic changes found in biofluids are anatomically or/and functionally connected with potentially disordered organ or system [3]. Estimation of the biosubstrata free and initiated crystallogenesis pecularities is highly significant because it is informative for clinical diagnostics, and isn't studied at all. The biosubstances are excrements and colon cleaning waters (colon lavage).
This research aim was investigation of saliva and colon cleaning water of the healthy people and patients under rectal detoxication.
Materials and methods
We tested free crystallogenesis properties of saliva and colon cleaning waters of the 12 healthy people and 12 patients after rectal ozone detoxication, which was accomplished by colon irrigation with the ozone-contained isotonic sodium chloride solution. Control points were in 1 hour after
After first procedure
After last procedure
Contact Information:
Д-р Мартусевич Андрей E-Mail: [email protected]
Fig. 1. The saliva and excrements crystalloscopic facias of the healthy people and patients under rectal irrigation of ozone-contained isotonic sodium chloride solution (x70)
the first and the last procedure.
The main crystalloscopic method was classic crystallo-scopy [3, 4]. Results evaluation of the own crystallization was accomplished by criteria system including structure index (SI), crystallization rate (CR), facials destruction degree (FDD) and marginal belt clearance (MB). We studied more than three micropreparation sight field .
Except visual morphometry we used spectrometric analysis of the crystals and amorphous structures formed after biological substrata dehydration. This method allowed to verificate the biological fluids morphology changes in the rectal ozonotherapy dynamics.
Statistic processing of the data was accomplished by Microsoft Excel 2003 spreadsheets and the programs Primer of Biostatistics Vers. 4.03. and SPSS 11.0. Results and discussion
By the crystallograms morphometric analysis (fig. 1) we determined that the first procedure of the rectal ozone-contained sodium chloride irrigation had a structurizing effect on dehydrated biofluids crystals. This was illustrated by the structure index. At the same time we noted temperate inhibition of crystallogenesis (on crystallization rate) connected with the basic level of marginal belt diameter (MB) and facials destruction degree growing (FDD). Similar changes were also found in colon cleaning waters but there crystallogenesis had a moderate character. It is necessary to underline that the crystal bodies were almost absent in the
Bulletin of the International Scientific Surgical Association
Vol.3, N. 1, 2008
specimens of the control group.
The course of rectal ozonotherapy has normalized all of the estimated parameters, but facials destruction degree of dehydrated saliva micropreparations was negative. In the excrements specimen we noted stabilization of the investigated criteria. They had an increased dynamics at last procedure (fig. 1).
The results of the biofluids spectrometric analysis demonstrated similar dynamics from the first to the last ozone-contained isotonic sodium chloride solution irrigations. We determined high correlation between spectrometric data and crystalloscopic estimation coefficients (|r| > 0,7; p < 0,05).
Conclusion
Biological fluids of gastrointestinal tract react actively on rectal ozone irrigations, and effects of one procedure and the whole course were different. It was determined that the course of rectal ozone detoxication had positive effect on the biosubstrata crystals building properties, and one procedure changed negatively some crystalloscopic characteristics.
References:
1. Dushov V. A., Kutnaya Gh. B., Baibulatova L. B. et al. Ozonotherapy effectiveness estimation in patients with chronic cerebro-vascular insufficiency on blood serum morphologic analysis. Nizhny Novgorod Medical Journal. 2005; Appl. «Ozonotherapy»: 79-81. (in Russian)
2. Gulyaeva S. F., Martusevich A. K., Pomaskina T. V. Math-ematic modeling of saliva initiated crystallogenesis results as a criterion of mineral water drinking effectiveness. Human ecology. 2005; 7: 33-35. (in Russian)
3. Martusevich A. K. Crystalloscopic investigation of biological substrata in pathological system determination on therapeutic and sergical gastrointestinal disorders. Gerald of Russian State Medical University. 2006; 2: 43-44. (in Russian)
4. Martusevich A. K., Kamakin N. F. Unified algorithm of biological fluids free and initiated crystallogenesis investigation. Clinical laboratory diagnostics. 2007; 6: 21-24. (in Russian)
5. Zubeeva G. N., Motyleva I. M., Potechina Yu. P. et al. Crystal-lographic screening in diagnostics and treatment of patients with artrial fibrillation. Clinical laboratory diagnostics. 2001; 9: 10. (in Russian)
А.К.Мартусевич, А.С.Лузан, А.В.Бочкарева
СВОБОДНЫЙ И ИНИЦИИРОВАННЫЙ КРИСТАЛЛОГЕНЕЗ БИОЛОГИЧЕСКИХ ЖИДКОСТЕЙ ПРИ РЕКТАЛЬНОЙ ОЗОНОТЕРАПИИ
Нижегородский научно-исследовательский институт травматологии и ортопедии, Россия
Аннотация:
Изучен характер свободной и инициированной 0,9%раствором хлорида натрия кристаллизации слюны и сыворотки крови 14 пациентов с термоингаляционной травмой. Оценены спектрометрические особенности новообразованных кристаллов биожидкостей при различных длинах волн в диапазоне 300-400 нм. Установлен тезиокри-сталлоскопический «паттерн» биосубстратов человека для рассматриваемого патологического состояния.
Ключевые слова:
кристаллизация, термоингаляционная травма, спектрометрия
© Н.Г.Можаровская, 2008
Н.Г.Можаровская
ПРИМЕНЕНИЯ ПУНКЦИОННОГО МЕТОДА ПОД СОНОГРАФИЧЕСКИМ КОНТРОЛЕМ В ДИАГНОСТИКЕ И ЛЕЧЕНИИ ЖИДКОСТНЫХ ОБРАЗОВАНИЙ ОРГАНОВ МАЛОГО ТАЗА
У ЖЕНЩИН
МЛПУ «Городская больница» г.МегионХМАО, Россия
Аннотация:
В работе приведен опыт использования лечебно-диагностических пункций под контролем эхографии при воспалительных и невоспалительных жидкостных образованиях малого таза у женщин. Ключевые слова:
воспалительные и не воспалительные жидкостные образования, малый таз
Актуальность проблемы.
В последние годы широко дискутируется вопрос о применении диагностических и лечебных пункций кист яичников и других образований малого таза под эхогра-фическим контролем. Интервенционная эхография в гинекологии ведет свое начало с 70-х годов прошлого века, когда H.H. Holm описал чрескожное пунктирование овариальных опухолей. В 1982 году D. Graham и R.C. Sanders предложили трансвагинальную аспирацию объемных образований малого таза под контролем трансабдоминального ультразвука. Ультразвуковой мониторинг нашел свое применение во многих интервенционных процедурах в гинекологии: пункции кист яичников с аспирацией и цитологическим исследованием полученной жидкости, пункции и дренирование образований
Contact Information:
Д-р Можаровская Наталья Георгиевна E-Mail: [email protected]
малого таза, таких, как абсцессы, послеоперационные гематомы, пункции опухолевых образований и лимфоузлов полости малого таза для диагностики рецидивов онкологических заболеваний после оперативного лечения. Доступ к органам малого таза всегда был затруднен в силу их анатомического расположения. Для решения этой проблемы было предложено несколько способов: трансабдоминальный чрескожный доступ, трансабдоминальный трансвезикальный доступ, трансректальный, трансперинеальный и трансвагинальный доступы. Наибольшее распространение получили ультразвуковой контроль из трансабдоминальной и трансвагинальной позиции. Для аспирации в настоящее время используются иглы с наружным диаметром 15-18 О (1,2 -1,8 мм). Цель работы. Показать доступность и эффективность метода.
Материалы и методы.
В МЛПУ «Городская больница» в 2007 году апробирован пункционно-дренажный метод в диагностике и лечении лечении жидкостных образований органов малого таза у женщин. Материал исследования составили
