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ACTA BIOMEDICA SCIENTIFICA, 2018, Vol. 3, N 5
КРАТКИЕ СООБЩЕНИЯ BRIEF REPORTS
DOI: 10.29413/ABS.2018-3.5.25 УДК 579.62, 579.61
Rodriguez I. ', Noda A.A. ', Fuentes O. Lienhard R. 2, Gern L. 3
Evidences about Human Tick-Borne Infections in Cuba
1 Tropical Medicine Institute "Pedro KourP' (Ave Novia del Mediodia, Km 6]h, La Lisa, La Habana, 11400, Cuba) 2Swiss National Reference Centre for Tick-Transmitted Diseases (Boucle de Cydalise 16, 2300, La Chaux-de-Fonds, Switzerland) 3 Laboratory of Eco-Epidemiology of Parasites, Institute of Biology, University of Neuchatel
(Emile-Argand 11, 2000 Neuchatel, Switzerland)
Serosurveys for IgG antibodies to Borrelia burgdorferi sensu stricto in a population exposed to tick bites (n = 247) and blood donors (n = 114) were done to assess the prevalence of tick-borne infections in Cuba. Seroprevalence of antibor-relial IgG antibodies was estimated in 0.6-7.2 % and 0 % of risk population and blood donors, respectively. While previous expositions to A. phagocytophilum (7.2 %), E. chaffensis (3.6 %) and B. microti (11.5 %) were serologically detected. These reports suggest the presence of tick-borne pathogens in Cuba, nonetheless lacking of further accurate information strongly calls to the need of more deeply studies.
Key words: Borrelia, Anaplasma, Ehrlichia, Babesia, seroprevalence, IgG, Cuba
For citation: Rodriguez I., Noda A.A., Fuentes O., Lienhard R., Gern L. Evidences about Human Tick-Borne Infections in Cuba. Acta biomedica scientifica, 3 (5), 164-165, DOI 10.29413/ABS.2018-3.5.25.
Распространённость клещевых инфекций среди населения о. Куба
Родригес И. Нода А.А. ', Фуэнтес О. Линхард Р. 2, Герн Л. 3
1 Tropical Medicine Institute "Pedro KourP' (Ave Novia del Mediodia, Km 6Уг, La Lisa, La Habana, 11400, Cuba) 2Swiss National Reference Centre for Tick-Transmitted Diseases (Boucle de Cydalise 16, 2300, La Chaux-de-Fonds, Switzerland) 3 Laboratory of Eco-Epidemiology of Parasites, Institute of Biology, University of Neuchatel
(Emile-Argand 11, 2000 Neuchatel, Switzerland)
Для оценки распространённости клещевых инфекций среди населения о. Куба были проведены серологические исследования на наличие специфических антител к Borrelia burgdorferi sensu stricto, Anaplasma phagocytophilum, Ehrlichia chaffensis and Babesia microti среди людей, пострадавших отукусов клещей (n = 247) и среди здоровых доноров (n = 114). Иммунная прослойка в отношении Borrelia burgdorferi sensu stricto составила 0,6-7,2 % среди группы риска и 0 % - среди здоровых доноров крови. Кроме того, были выявлены случаи контакта населения с A. phagocytophilum (7,2 %), E. chaffensis (3,6 %) и B. microti (11,5 %). Полученная информация указывает на возможность существования активных природных очагов трансмиссивных клещевых инфекций на Кубе. Для получения точной информации о распространённости клещевых патогенов в Республике Куба необходимы углублённые исследования.
Ключевые слова: Borrelia, Anaplasma, Ehrlichia, Babesia, иммунная прослойка, IgG, Куба
Для цитирования: Родригес И., Нода А.А., Фуэнтес О., Линхард Р., Герн Л. Распространённость клещевых инфекций среди населения о. Куба. Acta biomedica scientifica, 3 (5), 164-165, DOI 10.29413/ ABS.2018-3.5.25.
INTRODUCTION
Hard ticks (Acari: Ixodidae) are ectoparasitic arthropods and vectors of pathogenic microorganisms, affecting both humans and animals. The main species of hard ticks in Cuba are: Rhipicephalus sanguineus sensu lato, R. microplus, Dermacentor nitens and Amblyomma cajennense sensu lato; the last one is a three-host tick that feeds on a large spectrum of hosts, including humans. In our country, tick-borne infections in humans
have not been officially recognized due to the absence of direct manifestation of the causal agents in samples from clinical cases.
MATERIAL AND METHODS
Serosurveys for IgG antibodies to Borrelia burgdorferi sensu stricto in a population exposed to tick bites (n = 247) and blood donors (n = 114), and for Anaplasma phagocytophilum (n = 83), Ehrlichia chaffensis (n = 55)
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ACTA BIOMEDICA SCIENTIFICA, 2018, Том 3, № 5
and Babesia microti (n = 61) in people from the same population at risk were done. Sera from patients with clinical suspicion of Lyme disease during 1998-2016 were tested by ELISA-IgM/IgG and Western blot-IgM/ IgG for specific antibodies to B. burgdorferi sensu lato. Exploratory studies searching pathogens (Borrelia spp., Anaplasma spp., Ehrlichia spp., Babesia spp., Coxiella burnetii and Ricketssia spp.) on Cuban ticks mainly from horses, bovines and dogs have been conducted using Polymerase chain reaction, Reverse line blot hybridization and DNA sequencing.
RESULTS AND DISCUSSION
Seroprevalence of antiborrelial IgG antibodies was estimated in 0.6-7.2 % and 0 % of risk population and blood donors, respectively. Borrelial infection was also confirmed by specific IgM and IgG detection on clinically suspected patient sera. While previous expositions to A. phagocytoph-ilum (7.2 %), E. chaffensis (3.6 %) and B. microti (11.5 %) were serologically detected. DNA from Anaplasma/Ehrlichia spp., Babesia spp., R. amblyommii and Coxiella burnetii were detected in ixodid, mainly A. cajennense. These are the first reports suggesting the presence of tick-borne pathogens in Cuba, nonetheless lacking of further accurate information strongly calls to the need of more deeply studies.
Information about the authors
Rodriguez Islay - PhD, Researcher and Professor, Cuba Instituto de Medicina Tropical "Pedro Kourí" (Ave Novia del Mediodía, Km 6%, La Lisa, La Habana, 11400, Cuba; e-mail: islay@ipk.sld.cu)
Noda Angel A. - BSc, MSc, Cuba Instituto de Medicina Tropical "Pedro KourKourí" (e-mail: angelalberto@ipk.sld.cu) Fuentes Omar - Tropical Medicine Institute "Pedro KourKourí"
LienhardReto - MSc, Head of Departement; Swiss National Reference Centre for Tick-Transmitted Diseases, ADMED Microbiologie (Boucle de Cydalise 16, 2300, La Chaux-de-Fonds, Switzerland; e-mail: reto.lienhard@ne.ch)
Gern Lise - PhD, Director of Research, Laboratory of Eco-Epidemiology of Parasites, Institute of Biology, University of Neuchatel (Emile-Argand 11, 2000 Neuchatel, Switzerland; tel. +41 32-718-30-52, fax +41 32-718-30-01; e-mail: lise.gern@unine.ch)
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