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92Enterococcus gallinarum as a component of the Autoinfectome: the gut-liver-autoimmune rheumatic disease axis is alive and kicking
Dimitrios P. Bogdanos, Lazaros I. Sakkas
Mediterr J Rheumatol 2018;29(4):187-9
AN EDITION OF GREEK RHEUMATOLOGY SOCIETY AND PROFESSIONAL ASSOCIATION OF RHEUMATOLOGISTS
MEDITERRANEAN JOURNAL
OF RHEUMATOLOGY
E-ISSN: 2529-198X
mediterranean journal of rheumatology December 2018 | Volume 29 | Issue 4
mediterranean journal 29
of RHEUMATOLOGY 2018
GBogdanos DP, Sakkas LI.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International L
COMMENTARY
Enterococcus gallinarum as a component of the Autoinfectome: the gut-liver-autoimmune rheumatic disease axis is alive and kicking
Dimitrios P. Bogdanos, Lazaros I. Sakkas
Department of Rheumatology and Clinical Immunology, University General Hospital of Larissa, Faculty of Medicine, School of Health Sciences, University of Thessaly, Greece
Mediterr J Rheumatol 2018; 29(4):187-9 https://doi.org/10.31138/mjr29A.187
Article Submitted 02/05/2018, Revised Form 02/12/2018, Accepted 14/12/2018
Keywords: autoimmunity, autoimmune hepatitis, infection, microbiome, microbiota; systemic lupus erythematosus.
In recent years, we have repeatedly underlined the necessity to conduct meticulous assessments of the hierarchy of events that lead to the induction of autoimmunity
through the close and complex interaction of the micro-biota with a host who is prone to develop autoimmuni-ty.1-3 We recently introduced the concept of infectome and autoinfectome to describe those infections throughout life that cause autoimmune diseases in genetically susceptible individuals, who have impaired immunoreg-ulatory mechanisms.4-7 The events that take place before the establishment of infectious-related autoimmune disease largely rest on the microbiome changes of an individual over time. We proposed that environmental factors, which alter the normal microbiota may promote the growth of pathobionts which facilitate the development of autoreactive responses; such microbiota alterations
Corresponding author:
Dimitrios P. Bogdanos, MD, PhD
Associate Professor of Medicine and Autoimmune Diseases,
Department of Rheumatology and Clinical Immunology,
University General Hospital of Larissa
Faculty of Medicine, School of Health Sciences,
University of Thessaly
Viopolis 40500, Larissa, Greece
Tel.: +30 2413502880
Fax: +30 2413501016
E-mail: [email protected]
may also impact on the ability of the host to suppress autoimmunity due to their direct or indirect effect on the functional regulatory T and B lymphocyte populations.7 In a recent paper by Manfredo Vieira et al.8 published in Science, Enterococcus gallinarum, a Gram-positive gut pathobiont could translocate, under a state of a gut barrier breakdown, into a systemic organ (liver) and subsequently induce experimental autoimmune disease in genetically susceptible mice; namely, systemic lupus erythematosus. The translocating bacterium induced skewed T helper cell differentiation, and colonized the liver. These two main effects led to neoantigens and pro-inflammatory cytokines induction which promoted the production of autoantibodies and the development of autoimmune disease.
E. gallinarum had the potential for translocation in (NZW x BXSB) F1 mice but not in C57BL/6 mice under pathogen-free conditions. Thus, the authors tested translocation in germ-free C57BL/ 6 mice monocolonised with E. gallinarum.8 Their data were intriguing. When competing microbiota were absent, E. gallinarum was able to induce a) intestinal barrier leakage; b) de novo production of ANA (anti-dsDNA) autoantibodies, and c) translocation to mesenteric veins, mesenteric lymph nodes, and livers of non-autoimmune C57BL/6 mice. Of interest, no autoantibody production was seen in C57BL/6 mice during monocolonisation with other bacteria that either remained in the gut (B. thetaiotaomicron) or translocated to tissues (E. faecalis). In search of the immunological mechanism which perpetuate autoimmune phenomena, the researchers found that colonization with E. gallinarum alone induced Th17 cells in the small intestinal lamina propria and mesenteric lymph nodes of C57BL/6
Cite this article as: Bogdanos DP, Sakkas LI. Enterococcus gallinarum as a component of the Autoinfectome: the gut-liver-autoimmune 187 rheumatic disease axis is alive and kicking. Mediterr J Rheumatol 2018;29(4):187-9.
mediterranean journal 29
of RHEUMATOLOGY 2018
mice. These pro-inflammatory cells disappeared after vancomycin treatment. When this pathobiont was selectively depleted, through intramuscular vaccination using heat-killed E. gallinarum, mice exhibited reduced levels of serum autoantibodies and had prolonged survival. Such an effect was specific for E. gallinarum, as vaccination against E. faecalis or B. thetaiotaomicron did not influence autoantibodies or disease activity.8 Vaccination against E. gallinarum also prevented translocation. This was clearly documented by the lack of growth of E. gallinarum in internal organs.8
The fact that antibiotic treatment of bacterium-specific vaccination can cease autoimmune phenomena is a direct proof of the pivotal role of this specific microbe in the induction of autoimmune disease. As for all other infectious agents that have been directly linked to experimental autoimmune diseases, antibiotic treatment rather than immune suppression appears to be the proper treatment. These results were obtained in an experimental model of autoimmune disease. Arguably, the question arises if such data have any direct implication for the human disease. SLE patients have evidence of a 'leaky gut'; i.e. an impairment of gut barrier. When the authors assessed whether these patients have evidence of E. gallinarum translocation to human livers in patients with SLE (and concurrent autoimmune hepatitis [AIH]), 3 out of 6 SLE patients were positive for E. gallinarum, while none of the 6 controls obtained from healthy liver transplant donors with normal liver histology had evidence of the bacterium. These findings further strengthen the importance of this bacterium in the induction of the disease, SLE and/ or autoimmune hepatitis. The co-occurrence of autoimmune hepatitis and SLE is not a frequent phenomenon. However, several patients with autoimmune hepatitis have anti-dsDNA antibodies. It would be of interest to check those patients' livers for bacterium translocation. Most of the patients with newly diagnosed AIH have liver biopsies done at presentation for diagnostic purposes, and investigation in this biological material is feasible and rather easy to perform. AIH affects children, as well as adults, and extension of such an investigation to children may shed light on the hierarchy of the complex host-mi-crobiota interactions paying attention to E. gallinarum. A more relevant question would be if we need to search for evidence of E. gallinarum in all newly diagnosed patients with SLE, and if positive patients need treatment with antibiotics. In an animal model of a lupus-prone mice, vancomycin, initiated at 9 weeks of age, could attenuate lupus-like symptoms, reduce levels of IgG an-ti-dsDNA antibodies, diminish proteinuria, and decrease renal histopathological score. Such an effect was not seen with neomycin, an antibiotic with a broad spectrum of activity against both Gram-positive and Gram-negative bacteria. Vancomycin does not appear to trigger lupus-like disease in humans. However, vancomycin has
been considered a triggering factor of linear IgA bullous dermatosis, a rare autoimmune blistering disease and its exacerbating role in autoimmunity cannot be neglected. Though Enterococcus gallinarum is present in the normal flora in human and animal guts, the growing usage of broad-spectrum antibiotics increased the prevalence of infections caused by E. gallinarum, gradually leading to multi-drug resistance and nosocomial infections mainly affecting the urinary tract, abdominal and biliary tract;11 its ability to cause human autoimmunity remains to be seen. In conclusion, the recent findings of Manfredo Vieira et al.8 are rather provocative and need meticulous assessment in relevance to their implication in human disease. If E. gallinarum, a gut bacterium, is proved to be an important trigger of extra-intestinal autoimmune disease, we will need to reconsider the hypothesis of infectious-driven autoimmunity in the context of the autoinfectome.6,12
CONFLICT OF INTEREST
The authors declare no conflict of interest.
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ENTEROCOCCUS GALLINARUM AS A COMPONENT OF THE AUTOINFECTOME: THE GUT-LIVER-AUTOIMMUNE RHEUMATIC DISEASE AXIS IS ALIVE AND KICKING
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