CC BY
5*
ALT'23 The 30th International Conference on Advanced Laser Technologies
B-I-36
Diagnostics of porous materials and biological tissues via diffusion-associated strain measurement with OCE
Yu.M. Alexandrovskaya1, O.I. Baum2, E.M. Kasianenko2, A.A. Sovetsvky1, A.L. Matveyev1,
L.A. Matveev1, V.Y. Zaitsev1
1 Institute of Applied Physics of the Russian Academy of Sciences, 603950 Nizhny Novgorod, Russia 2 Institute of Photon Technologies, Federal Scientific Research Centre 'Crystallography and Photonics' ofRussian
Academy of Sciences, Troitsk, 108840 Moscow, Russia
In this study we investigate diagnostic capabilities of optical coherent elastography (OCE) using visualization of osmotically induced slow deformations (tens of minutes) in porous water-saturated polymers and biological tissues [1,2]. Diffusion of hyperosmotic liquids and optical clearing agents through these materials is accompanied by subsurface strains, spatio-temporal evolution of which (amplitude, sign and rate) can be monitored in real time by OCE. It has been shown that the analysis of the dynamics of osmotically-induced deformation can be used to diagnose the presence of pathologies, such as the degradation of proteoglycans in cartilage tissue. The rate and amplitude of osmotically induced shrinkage and dilatation in polyacrylamide gels have been found to be dependent on the degree of their crosslinking [3]. Some possibilities of observing the development of crosslinks in tissues under the action of cross-linkers, such as glutaraldehyde, in real time have also been demonstrated. It is discussed how the formation of crosslinks depends on the initial composition and concentration of the used solution.
The study was supported by the Russian Science Foundation grant No. 22-12-00295
[1]. Alexandrovskaya, Y.; Baum, O.; Sovetsky, A.; Matveyev, A.; Matveev, L.; Sobol, E.; Zaitsev, V. Optical Coherence Elastography as a Tool for Studying Deformations in Biomaterials: Spatially-Resolved Osmotic Strain Dynamics in Cartilaginous Samples. Materials 2022, 15, 904. https://doi.org/10.3390/ma15030904
[2] Yu. Alexandrovskaya, O. Baum, V. Zaitsev, A. Sovetsky, A. Matveyev, L. Matveev, K. Larin, E. Sobol, V. Tuchin, Optical and mechanical properties of the cartilage during optical clearing, In book Tissue optical clearing: new prospects in optical imaging, CRC Press (Dan Zhu, Elina Genina, and Valery Tuchin Eds.), 2021, Boca Raton, Florida, United States.
[3] Alexandrovskaya, Y.M.; Kasianenko, E.M.; Sovetsky, A.A.; Matveyev, A.L.; Zaitsev, V.Y. Spatio-Temporal Dynamics of Diffusion-Associated Deformations of Biological Tissues and Polyacrylamide Gels Observed with Optical Coherence Elastography. Materials 2023, 16, 2036. https://doi.org/10.3390/ma16052036.
