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Abstracts. PHYTOPHARM 2017
2.5 g extract granules 3 times daily (inacceptable for European patients), we were able to develop a novel dry extract for pelletizing, thus concentrating the common Japanese daily dose of secondary metabolites from Shikunshito in just 3 tablets per day (Convenience!). Due to the fact that Shikunshito is used in the EU in
single prescription by Kampo doctors since more than 15 years and since centuries in Japan without negative effects, the presented work opens the possibility to apply for its official registration as a phytopharmacon in Europe.
DEVELOPMENT OF A NOVEL PROANTHOCYANIDIN ENRICHED GINKGO BILOBA L. LEAVE EXTRACT WITH IN VITRO AND IN VIVO NEUROPROTECTIVE EFFECTS
© KuchtaK.1, Qiao H.X.2, Huang H.B.2, Fang L.2, Chen Y.3, Wang R.W.24
1 National Institute of Health Sciences, Division of Pharmacognosy, Phytochemistry & Narcotics, Tokyo, Japan;
2 Zhejiang CONBA Pharmaceutical, Hangzhou, China;
3 Zhejiang University of Technology, Hangzhou, China;
4 Zhejiang Provincial Key Laboratory of TCM Pharmaceutical Technology, Hangzhou, China
A commercial refined extract of Ginkgo biloba L. leaves has been reported to protect brain tissue against cerebral ischemia/reperfusion (I/R) injury [1]. This activity is generally attributed to the antioxidant activity of its flavonoids and proanthocyanidins. However, the latter have not been adequately studied up to now. Therefore, the present study aims to assess the neuroprotective properties of a newly developed proanthocyanidin enriched G. biloba leave extract (GPE) - with >90% proanthocyanidins after resin adsorption - on cerebral I/R injury compared to commercial proanthocyanidins from grape seeds (Vitis vinifera L.) (GSP) and nimodipine as a positive control. In vitro, the tissue protective effect of GPE was measured using PC12 cells, cultured in 96-well plates. After the addition of GPE or GSP (0.625, 1.25, 2.50 ^g/ml) and 12 h of cultivation, cells were incubated with H2O2 (40 mM) for another 24 h. Cell damage was measured using a commercial LDH assay kit. GPE noticeably ameliorated the increase in LDH release and thus the respective decrease in cell viability. For the in vivo assay, male Sprague-Dawley rats were divided into 6 groups
(sham: 8, placebo: 25, GPE 80 mg/kg: 13, GPE 40 mg/kg: 13, GPE 20 mg/kg: 16, GSE 40 mg/kg: 18, nimodipine: 8). All non-sham animals were subjected to cerebral I/R injury by occluding the middle cerebral artery with a nylon suture that was removed after 2 h of ischemia to establish reperfusion. After recovery from anaesthesia, the rats were returned to their cages with free access to water and food. All animals were sacrificed 24 h after reperfusion. Coronal brain sections were stained in order to calculate infarct ratio. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels in the brain tissue were measured using commercial assay kits. Under treatment of cerebral I/R injury in test animals with GPE, the death rate decreased, neurological dysfunctions were reduced, and both average infarct size and concentrations of MDA and SOD were significantly ameliorated as compared to the placebo group.
References:
1. Hu B, Sun S, Mei G, Chen L, Tong E, 2002. Chin Med J (Engl), 115(9):1316-1320.
CURRENT PROGRESS AND FUTURE PROSPECTS OF ANTRODIA CINNAMOMEA RESEARCH IN TAIWAN
© K.J. Senthil Kumar1, Sheng-Yang Wang12
1 Department of Forestry, National Chung Hsing University, Taiwan;
2 Agricultural Biotechnology Research Center, Academia Sinica, Taiwan
Antrodia cinnamomea (Syn. Antrodia camphorate or Taiwanofungus camphorates) is a unique medicinal mushroom endemic to Taiwan. In traditional Chinese medicine (TCM), A. cinnamomea is used for treating various human illness including, food poisoning, drug intoxication, diarrhea, abdominal pain, hypertention, skin irritation, inflammation and cancer. In natural habitat, this mushroom grows the inner sap of age old camphor tree
Cinnamomum kanehira Hay (Lauraceae). Accumulating scientific evidences (nearly 400 research articles) revealed that A. cinnamomea possess various therapeutic effects including, immunomodulation, hepatoprotectiion, neuroprotection, anti-oxidant, anti-inflammation, antihypertensive, anti-hyperlipedemic, anti-diabetic, anti-metastatic and anti-cancer activities. In recent years, this mushroom is starting to attract by pharmaceutical
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
vol. 15/2017/suppLement 1
