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Determination of microrelief of the sample by singular beams superposition
B. Sokolenko1, N. Shostka2, O. Karakchieva 2, A.V. Volyar1, D. Poletayev1 1 V.I. Vernadsky Crimean Federal University, Institute of Physics and Technology, 295007, Vernadsky av., Simferopol, Russia, 2 V.I. Vernadsky Crimean Federal University, Scientific Research department
Abstract
In present paper we propose easy way to implement method of interfering vortices with opposite topological charge for the real time determination of the thickness and information about the surface of studied samples with the resolution up to 7 nanometers. The determination of the characteristics of the medial cross-section of submicron-objects becomes possible due to phase sensitivity of interfering singular beams to the slightest changes in the optical path difference between them. The dependence of rotational angle of resulting interference pattern in case of different sample thickness for two singular beams superposition is considered in detail.
Keywords: optical vortex, phase, optical microscopy, singular beams, surface relief detection.
Citation: Sokolenko B, Shostka N, Karakchieva O, Volyar AV, Poletaev D. Determination of microrelief of the sample by singular beams superposition. Computer Optics 2019; 43(5): 741-746. DOI: 10.18287/2412-6179-2019-43-5-741-746.
Acknowledgments: This work was supported by the Russian Foundation for Basic Research (RFBR) and the Council of Ministers of Crimea grant № 19-42-910010, № 17-42-92020 and partially supported by the V. I. Vernadsky Crimean Federal University Development Program for 2015 - 2024 and Foundation for Assistance to Small Innovative Enterprises (Russian Federation) (Grant №11540GU/2017 (0033028). The results of the work were reported and discussed at the international conference "Digital singular optical optics", September 17-21, 2018, Sevastopol.
Introduction
Intensive developing of nanotechnologies in electronics, engineering, biology and biomedicine needs new techniques for fast, low-error and easy in realization measurements and increasing the resolution of existing methods of optical microscopy. Thus, in order to meet the new needs of applied sciences a large number of recent publications in the field of modern optics were devoted to the problem of beam reflection or its propagation through the studied samples for both: coherent and non - coherent light sources [1-3] in order to develop methods for non-contact study of micro- and nanostructures in material processing as well as biomedical applications [4].
One of the most common principles of optical microscopy - scanning probe microscopy, is an area of microscopy in which the image of the object surface formed by a probe scanning its surface. The image obtained by mechanically moving the probe along a path in the form of a raster (line by line) and registering the interaction between the probe and the surface as a function of its position. This type of microscopy includes Scanning Near-field Optical Microscopy (SNOM), Atomic Force Microscopy (AFM), and Scanning Tunneling Microscopy (STM) and vide range of Fluorescence Microscopy modifications [5-8].
Furthermore, Stimulated Emission Depletion Microscopy (STED), a type of fluorescence microscopy that achieves a resolution in excess of the diffraction limit by selective fluorescence quenching, can be distinguished [9]. An important advantage of STED microscopy is that the resolution improvement achieved in real time and usually does not require long-term data processing. Also, a newest application of "doughnut" beam carrying tightly focused optical vortex by high numerically aperture objective was implemented in STED microscopy [4, 8-11].
Optical methods of surface analysis, which use an in-terferometry and coherent light sources, have many advantages, including non-contact and non-destructive interaction with the test sample [12, 13], high resolution [14], etc. The main difficulties of such method are high sensitivity to vibration and speckle noise. The salvation of peculiarities mentioned above leaded to the searching of methods based on using of Bessel and Laguerre-Gaussian beams. [15, 16] where the dependence of rotation angle for intensity distribution of so-called "two-lobed fields" were investigated in dependence on the depth of focus for spiral beams as well as influence of amplitude and phase distortions on the formation of the light field with the intensity rotation was established. [17]. Thereby, in latest research [18] authors proposed a new concept of full-field topographic microscopy based on array of self-interfered optical vortices enabling a reference-free displacement and 3D shape measurement of reflective samples due to a very high rotation sensitivity to defocusing which can be applied to an accurate depth estimation in conventional optical microscope [19, 20]. Another method named Optical Vortex Scanning Microscope for visualization and analysis of nanometer structures, where the sample is scanned by a moving vortex, was demonstrated in work [12]. Hence, the way of investigations the surface roughness and the thickness of the sample were demonstrated in work [21] by analyzing interference patterns in the form of a vortex spiral. The interference patters between reference beam and object vortex beam passing through the sample demonstrates rotation depending on surface parameters of the sample. In contrast to [17-19] probe beam contain single charged optical vortex which interfered with Gaussian beam and rotation of interference pattern analogues to "two-lobed
fields" allow to define surface height difference by angular orientation. The method has shown good results due to the high sensitivity of singular beam phase to the small distortions of the wave front caused be the sample. However, one of the significant drawbacks should be indicated as the complexity of the determination the rotation of the interference spiral with sufficient accuracy.
In this paper, we propose an optical scheme of investigation of the sample profile with nanoscale resolution based on the analysis of evolution of the interference pattern of two superimposed vortices with single topological charges and opposite signs. This method is suitable for the real time determination of the thickness and information of surface of studied samples. The determination of the characteristics of the medial cross-section of sub-micron-objects becomes possible due to phase sensitivity of the singular beam to the slightest changes in the wave field. The dependence of the angle of rotation of the resulting interference pattern of two singular beams on the sample thickness is considered in detail.
1. Theoretical basis
The phase and propagation characteristics of Laguerre-Gaussian (LG) beam are of great current research interest and found an application in various optical devices for trapping, moving and angular orientation of microparticles in combination with Bessel beams as well [22-31]. The last significant achievements in the research were implemented in relation to the practical application of the LG beams, in particular, in microscopy. A large number of publications in recent years are devoted to the problem of beam reflection or its propagation through the studied samples for coherent and non-coherent light sources in order to develop metrological methods for non-contact study of nanostructures. These principles can be used for investigation the surface profile of samples of different nature with high resolution [11-18]. Summarizing, Laguerre-Gaussian beams carrying low-order optical vortex is the most convenient as a probe beam for development of phase retrieval methods in application to metrology of optically transparent and reflecting samples, which may have not only sophisticated surface relief but also internal structural inhomogeneity.
Laguerre-Gaussian beam is the solution of the wave equation with radial symmetry of the intensity distribution. The simplest type of Laguerre-Gaussian beams is an optical vortex (when the radial index is zero) possessing a phase singularity and characterized by the preservation of the zero intensity on the phase uncertainty line in the par-axial beam, as well as the ability to transfer its own orbital angular momentum.
A particular solution of the paraxial wave equation for the LG beam can be written as follows:
E = — w
in: \ H f 0 2 > f 2 >
2r 2
• exp
<exp
V / V / V / ..2 >
ikr 2
• exp (-i/p) >
2 z (1 + z2R/z 2), exp (i(2w +1 -1) arctan (z/zR )) • exp(-ikz),
(1)
where zR = kra 0 /2 determines the Rayleigh length, rao -beam waist in the plane of incidence on the surface of the sample z = 0, l=+1 - topological index,
w = ra0^1 + (z/zR )2 - current beam waist, 9 = arg(x + iy) -
azimuthal angle, r = yjx2 + y2 , k=n • (2tc /X)- wave number in the medium with refractive index n.
Thus, the main parameters affecting the quality of measurement are waist radius, Az - geometrical path difference, A9 - phase difference caused by various thickness of explored sample and n - refractivity index for transparent materials. We restrict ourselves on linearly polarized paraxial Laguerre-Gaussian beams with cylindrical symmetry to avoid undesirable intensity downturn and structural instability [32]. As it was found by numerical simulation and confirmed experimentally in [33], the spreading of low-order Laguerre-Gauss beams carrying optical vortex through a random distorting medium experiencing a minimal structural deformation, thus choosing of vortex with topological charge l=+1 is practically justified.
As shown in the paper [34] interference of two La-guerre-Gaussian beams carrying equal but opposite topo-logical charge resembles two slices of maximum intensity, separated by a thin dark band corresponding to the minimum intensity.
Computer models of the transverse intensity and phase distributions in the singular beams, as well as the result of their interference, are presented in Fig. 1.
Phase images can be considered as a two-dimensional phase distribution or as an optical path difference of interfering rays. For visualization of optically inhomogeneous transparent media, it is known that the dependence of the optical path difference is proportional to the local projection of the refractive index on the beam direction:
h (x, y) =
1
n ( x,y,z)
where X - the wavelength of the incident wave.
The main task is to analyze the dynamics of the recorded intensity when the phase difference of the interacting beams changes. When the optical paths of two beams in the arms of the interferometer are close, the interfering waves have the same radius of the beam waist rao and the curvature of the wave front. Only in this case we may obtain symmetrical intensity distribution of superposed singular beams.
The method of determining the angle of rotation for the interference pattern is quite simple. The program determines the center of the dark band between two slices of maximum intensity, through which a line is drawn as shown in Fig. 2, with respect to which the rotation of the similarly constructed line of the following interference pattern is determined when the optical path difference of the interfering beams changes, corresponding to the elevation of the sample.
Thus, the analysis of the changes in the obtained interference patterns allows us to extract information about the phase shift between the superimposed beams and the state
of surface roughness and thickness of the sample, as well as the problem of optimizing such procedures in practice.
In practice, it is possible to use two types of measurement schemes: when the singular beam is a probing
beam and when the vortex is introduced into the reference beam. In both cases, the interference patterns are the same, but the direction of their rotation will be inverted.
Fig. 1. Numerical model of LG beams superposition with opposite topological signs: (a) intensity distribution (inverted) of LG beam with unit positive topological charge and opposite one (b) respectively; (c) and (d) - the pairs ofphase maps corresponds to the phase patterns of optical vortices (LG beams with zero radial index) and opposite topological charges; (e) is the resulting pattern of superposition of two LG beams of equal and opposite by the sign topological charges and (f) corresponds to the phase portrait of interfered singular beams. Parameters of superimposed beams were the following: rn = 180 /m, n = 1.00, z = 1 m, 1 = 632.8 nm
Fig. 2. Interference patterns (inverted) of pair (a) The angle between symmetry axes 36,5° is
2. Phase-shift retrievals for transparent threads: an experiment
Due to its high sensitivity to wave front inhomogenei-ty, caused by geometry of the sample, optical vortices and their interference was adopted to surface roughness analysis and curvature imaging of reflecting surfaces [2, 21]. Nevertheless, transparent samples with complex relief of boundary surface may have an inhomogeneity of refrac-tivity index. Both these parameters define the overall optical path difference between object and reference beams. From this point of view, such samples are amplitudephase objects. The simplest way for fast and computationally-non-intensive analysis of such objects should be used in form of direct data acquisition from image sensors and cameras with minimal adjustments and transformations. For this purpose, a superposition of a high-sensitivity probe beam carrying optical vortex with a reference beam also containing a singularity with the opposite sign was used. To estimate the sensitivity of two-singular beams interference method we restrict ourselves on consideration only homogeneous isotropic media as, for example, thin cover slip. Typical cover slip has 250 ^m thickness and high quality of parallelism. In case, when the singular beam slide on the isotropic plate with a gradient surface profile, we can choose an arbitrary posi-
and (b) neighboring positions with A = 128.0 nm. well observed after digital processing (c)
tion and set it as starting point, thus all the next measurements will be written relatively to the value of phase-shift at this point in terms of angular turn of intensity pattern. This effect becomes the basis for a high-resolution microscope device, where rotational process may be easily captured experimentally and processed automatically in real time.
Typical experimental set-up for two-beam interference includes vortex beam shaping unit and interferometer with almost equal distances in object and reference arms to avoid beam waists misalignment. The scheme, proposed in Fig. 3c, allow to control test object position and very optical path difference between the beams. The light beam from the He-Ne laser is converted into the single-charged vortex beam by computer generated hologram H with "fork"-shape amplitude produced by photographic method [35-37]. The first diffraction order was cut by the diaphragm D and five-times expanded with telescope T. Next the telescope T beam splitter BS1 splits the initial vortex beam with topological charge l=+1 to the object and reference beams, the reference beam has opposite sign of charge l = -1 for receiving of appropriate interference pattern on the CCD camera. Microscope objectives MOi and MO2 10x with numerical aperture 0,30 focused the beam on the sample surface and project the
light on camera matrix. Both microscope objectives adjusted so that to receive parallel light beam after the sample without scale transformation. Taking into account that polarization state has greatest influence to the longitudinal component of the electric vector of the light field, thus the greatest visual difference in the intensity picture is observed when using the first-order vortex, which was demonstrated in [38, 39].
Fig. 3. Schematic representation and the principle of thickness measurement for transparent object: (a) rotational sample holder SH with the cover slip S of thickness d = 250 ¡um; (b) principle of metering, where two measurements were token: first one "1", when slip was placed strictly horizontally and the second one "2" with tiny inclination (a= 0+5") controlled with
sample holder SH; (c) optical configuration of experimental set-up: the Gaussian beam emitted from He-Ne laser converted into the Laguerre-Gaussian, which is carrying optical vortex with computer synthesized hologram H and filtered by diaphragm D after what the first diffraction order with topological charge l = +1 was separated and 5x expanded by telescope T. The Mach-Zehnder interferometer was built on pairs of beam splitters BSi and BS3 and mirrors Mri, Mr2. An
extra beam splitter Bs2 in the reference beam serves for inversion of optical vortex topological charge to opposite one on the exit from interferometer (l = -1). Microobjectives MOi and MO2 focus the beam on the sample surface and project the light on CCD camera and stored
Such wise, we choose focusing conditions for operating in paraxial regime, where longitudinal components of electric field and their contribution to the intensity pattern may be neglected. The length of interferometer arms is 500 mm and was chosen equal as possible, the mirror Mri was inserted for realization of even number of reference beam reflections and keeping opposite sign of topo-logical charge. Other distances between optical elements are not critical, so the setup scheme may be easily transformed for measurement of reflecting samples.
Slightly inclined test sample makes possible to control the effective optical thickness, which causes on phase shift between the beams. This process is observed visually by turn of interference pattern, as shown in Fig. 4. Stabilization of interference pattern was reached by synchronization of probe beam and reference one with the sample itself. Such principle allow avoiding instability of interference pattern caused by random movement and shudder. Moreover, proposed method is resistible against the aberrations of optical elements since only central part of the beam carrying necessary information about vortex phase.
Fig. 4 shows the dynamics of interference pattern for different values of optical path difference. The intensity minima correspond to unfolded phase profile of superimposed beam and may be easily recognized with computer vision procedures. The geometrical thickness of the sample under test is defined as optical path difference divided by refractivity index n of glass, where n = i.5168.
5cp=0° 8=0 nm
dv?=43° 8=151 nm
Sep=90° 8=316nm
dep=135° 8=474 nm
dq>=180° 8=633 nm
Fig. 4. The evolution of superposition of vortex beams with opposite signs of topological charge for different optical path differences z+S. (a) Experimental patterns (inverted) and (b) corresponds to computer simulation of intensity distribution (inverted), (c) shows the evolution the phase of resulting field Im E(r, ))
An estimation of sensitivity for turn of superposition pattern indicates that the resolvable threshold for determining the rotation angle of the pattern is 3" for current state of the experimental set-up. One full turn occur, when optical path difference acquires the value of a multiple of the wavelength X, for He-Ne laser (X = 632.8 nm) the vertical resolution is S = 10.54 nm. In dependence of media refractivity index n ^ 1, the total resolution for
physical thickness measurement may be expressed as Sgeom = 10.54/n and reaches for glass material (BK-7) with n = 1.5168 Sgeom = 6.95 nm.
The total phase shift observed by the angular rotation of the interference spiral was calculated using simple expressions: dq> = 0 -r- 2n, mX = n (d1 - d2) = nh, where d1 - d2 - is the height difference between two adjacent levels of the sample surface (geometric path), mX - the optical path difference with multiply factor m and dq> - is the phase-shift caused by difference in thickness. A complete depth map can be compiled by step-by-step scanning in the xy plane by the sample holder transverse movement, so that a sample may be examined and imaged without limitations to linear dimensions.
Conclusion
Development of new methods for phase shift analysis in application to thickness measurement is the quite important issue in fundamental and applied research in optical noninvasive metrology. A great attention paid to the development of optical methods is caused, first of all, by the fact that in field of applicable measurement technics in nano-research and the nano-systems industry is not limited from the point of view of image quality improving, but also has great importance for such tasks as recording information in the optical environment, lithography, nano-structuring and optical manipulation. In particular, great attention was attracted by the possibility of using interferometric methods for investigating stepped structures in industry and, as result, may be applied to interdisciplinary research as bio-photonics and medicine.
This research presents the results of theoretical analysis, simulation and experiment, the sensitivity of singular beams to external perturbations is determined. It is shown that a discernable rotation of intensity profile arises during the changes of sample thickness with step of 6.95 nm. The proposed technology is used in the microscopy of optically transparent and reflective media, where the method benefits from the undemanding to optical elements quality and distances between them in the experimental setup. This approach enable to scale it for successfully deploying in the measurements of stepped structures as thin films, coatings and roughness of substrates in noncontact operation regime, as well as in area of biopho-tonics and crystal optics. Thus, proposed imaging method is performed non-invasively on intact, fully functioning organisms, real time microscopy allows the study of development in evolution.
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Authors' information
Bogdan V. Sokolenko (b. 1987), graduated from Taurida National V.I. Vernadsky University in 2010. Entered in postgraduate study in 2013 on specialty 01.04.05 "Optics", finished his education in 2013. He received the PhD degree in Physics in 2015. Candidate in Physics and Mathematics. He works on General Physics department of Institute of Physics and Technology of V.I. Vernadsky Crimean Federal University (Simferopol) as an associate professor. Also, he is researcher of Singular Optics laboratory at the area of microscopy, optical systems construction, anisotropic media and crystals. He is co-author of 14 papers indexed by Scopus and 3 inventions. E-mail: [email protected] .
Natalia V. Shostka, (b. 1987), graduated from Taurida National V.I. Vernadsky University in 2007 (now V.I. Ver-nadsky Crimean Federal University). She received the PhD degree in Optics, Laser Physics in 2013. Candidate of Physical and Mathematical Sciences. Research interests: singular optics, polarization and phase transformations, optical trapping and manipulation, tight focusing. E-mail: [email protected] .
Olga S. Karakchieva (b. 1987), graduated from Taurida National V.I. Vernadsky University in 2010. Entered in postgraduate study in 2013 on specialty 01.04.05 "Optics", finished his education in 2014. She received the PhD degree in Physics in 2014. Candidate of Physical and Mathematical Sciences. She works in Scientific department of V.I. Vernadsky Crimean Federal University. Area of scientific interests: coherent and nonlinear optics; co-author of 9 papers indexed by Scopus and Web of Science. E-mail: olga.karakchieva@gmail. com.
Alexander V. Volyar (b. 1949) graduated from Simferopol State University (1972), and after graduation - his employee. Doctor of Physical and Mathematical Sciences (1992). Received his degree of the PhD of Technical Science in the field of Physical and Quantum Electronics in the Moscow Institute Radio-Electronics in 1981 and his degree of the Doctor of Physical and Mathematical Science in the field of optics and laser physics in The Institute of Physics of Ukraine Academy of Science in 1992. Since 1993 he worked as professor of General Physics department and since 1999 - the Head of General Physics department. Volyar A.V. is the author more than 170 scientific articles in the field of nonlinear optics, fiber and singular optics. Area of scientific interests: singular optics and laser physics. One of the founders of a new direction in physics - singular and topological optics. E-mail: [email protected] .
Dmitrii A. Poletaev (b. 1984) graduated from Taurida National V.I. Vernadsky University in 2006, works as an as-sosiate professor of Radiophysics and Electronics department Crimean Federal University (Simferopol). Research interests: microwave, microwave diagnostics, nanotechnology, programming, robotics. E-mail: [email protected] .
Received October 5, 2018. The final version - June 28, 2019.
