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14set of data said that the comparison between groups using t test, p<0.05 was statistically significant. Experiment results
Results of FOXC1 and FOXC2 protein expression in lung tissue ( ±S)
Group Grayvalue of FOXC1 Grayvalue of FOXC2
(/GAPDH) (/GAPDH)
Blank group 0.258±0.013 0.094±0.008
Model group 0.544±0.030* 0.184±0.010*
Dexamethasone group 0.444±0.021*# 0.118±0.004*#
Pingchuan Granule group 0.453±0.024*#^ 0.123±0.006*#^
Note: Compared with the blank group *p < 0.05; compared with the model group#p < 0.05; and compared with the dexamethasone group^ P > 0.05.
4. Discussion The experimental results showed that the Pingchuan Granule might affect inhibition of the DLL4/ Notch signaling pathway upstream factor expression and regulation of DLL4/Notch signaling pathway to improve angiogenesis / vascularremodeling and pathological changes of the airwayto treat of asthma.
References :
[1]Li D, Yan D, Liu W, et al. Foxc2 overexpression enhances benefit of endothelial progenitor cells for inhibiting neointimal formation by promoting CXCR4-dependent homing - Journal of Vascular Surgery[J]. Journal of vascular surgery, 2011, 53(6):1668-78.
[2]Hayashi H, Kume T. Foxc transcription factors directly regulate Dll4 and Hey2 expression by interacting with the VEGF-Notch signaling pathways in endothelial cells.[J]. Plos One, 2008, 3(6):952-952.
COMPARATIVE STUDY ON ANTI-TUMOR OF HEDYOTIS DIFFUSA HERBA, SCUTELLARIAE BARBATAE HERBA COMPATIBILITY ANGELICAESINENSIS DECOCTION FOR SUPPLEMENTING BLOOD
Wang Yanjie Kang Xinrong LiJi Sun Yang Liang Ying
(Heilongjiang University Of Chinese Medicine,Harbin 150040,China)
Author:Wang Yanjie (Email:[email protected] Universi-
ty Of Chinese Medicine,Harbin 150040,China); Correspondence author:LI-
Ji (Email:[email protected] University Of Chinese Medicine,Harbin 150040,China)
Objects:Study on the molecular mechanism of hedyotis diffusa herba(HDH) &scutellariae barbatae herba(SBH) with heat-clearing and detoxifying "elimination method " anti-tumor, and angelicae sinensis decoction for supplementing blood(ASDSB) with fuzheng guben "supplementation method" anti-tumor,and two together with "elimination and supplementation method" anti-tumor, provide relevant experimental study for using drugs to form a prescription on clinic, and to lay the foundation for research and development of new clinical preparation.
Materials and methods:Based on animal models,got tumor-bearing mice serum and tumor tissue as materials to carried out the experiment: (1)Determined the cytokines levels of IL-2 ,TNF-a and INF-y in serum by double-antibody enzyme-linked immunosorbent assay(ELISA) to analysis anti-tumor immunological mechanisms of HDH-SBH,ASDSB,and the two together;(2) Used the HE staining to observe themorphological changes of tumor tissue ;(3)Used western blotting to observe the expression of JaK2,STAT1 in tumor tissue ,to look for the anti-tumor mechanism of signal transduction.
Results and discussion:
1.The tumor inhibition rate of HDH-SBH was as high as 50.82%; The tumor inhibition
rate of ASDSB was 41.28% ; The tumor inhibition rate of two together group (four in one )Was 43.12%.
2.The effects of different Chinese medicine groups on the immune organs of S180 tumor bearing mice could promote the activation of immune cells and the secretion of cytokines.
3.The HDH-SBH group could increase the the content of TNF-a, INF-y, IL-2,the ASDSB group could increase the content of IL-2 , and the two together group (four in one )could increase the content of IL-2,TNF-a in serum of tumor bearing mice (P<0.05) .
4.Three groups of traditional Chinese medicine on EGF receptor mediated signal transduction pathway with different degrees of inhibition have been accomplished by reducing phosphorylation levels of JaK2, Stat1 in tumor tissue.
References:
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Амурский медицинский журнал №3 (19) 2017
[1]LIU Songjiang ,HAN Shuli,ZHU RIqi,DUAN FUji.elimination and supplementation method in the application of the malignant tumor treatment[J].Acta
Chinese Medicine and Pharmacology,Vol.42,No,2,2014,42-44 pages.
[2]Yuan-ChiehYeh,Hsing-YuChen,Sien-HungYang,Yi-Hsien Lin, Jen-HweyChiu, Yi-Hsuan Lin,and Jiun-LiangChenl.Hedyotis diffusa Combined with Scutellaria barbata Are the Core Treatment of Chinese Herbal Medicine Used for Breast Cancer Patients:A Population Based Study.Evidence-Based Complementary and Alternative Medicine.
Volume 2014, Article ID202378,9pages.
[3]WANG Qunhong, HU Min, XIANG Xueyan, WANG Guanfu.Experimental Study
on Anti — tumor Effect of Danggui Buxue Decoction[J].Chinese Archives of Traditional Chinese Medicine,Vol.32,-No.4,2014:904-905+974.pages.
THE EXPERIMENTAL STUDY OF THE EFFECT OF THE ACUPUNCTURE THERAPY OF ACTIVITING BRAIN AND REGAINING CONSCIOUSNESS ON THE EXPRESSION OF PROTEINS RELATED TO NEURONAL APOPTOSIS IN THE HIPPOCAMPUS TISSUE OF ALZHEIMER'S DISEASE RATS
Wang Yujue1 ; Lv Ling2
(1.The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150001, China ; 2.The Second Hospital of Harbin, Harbin 150001, China)
Biography : Wang Yujue(1985.01-), Master Degree Candidate, Research Direction : To treat nervous system disease by acupuncture combined with rehabilitantion, Mailing Address : Heilongjiang province Harbin city Nan-gang District Gogol Street No. 411, the doctor's office of third rehabilitation ward of the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Mailbox : [email protected], Tel : 13100880662.
Corresponding Author : Lv Ling(1984.01-), Master Degree Candidate, Research Direction : To treat senile disease by acupuncture therapy, Mailing Address : Heilongjiang province Harbin city Daowai District Satellite Road No. 38, the doctor's office of occupational disease ward of the Second Hospital of Harbin, Mailbox : [email protected], Tel : 13766821932.
The Alzheimer's disease, also named senile dementia[1], is a primary neurodegenerative disease of brain[2]. Its main clinical symptoms are recent-memory disturbance, decreasing of learning ability, l-apopathy and dyskinesia[3]. The morbidity of the Alzheimer's disease went up year by year[4]. Older age and lower level of education were highly correlated with the development of AD[5]. Thus, finding an effective method to control the Alzheimer's disease is the major task to be solved in the world.
Objective: This study aims to explore the effect of the acupuncture therapy of activiting brain and regaining consciousness on the learning and memory function and the expression of Bcl-2, Bax and Caspase-3 in the hippocampus tissue of Alzheimer's rats mediated by AP1-42. Investigate the mechanism of the anti-apoptotic action of the acupuncture therapy of activiting brain and regaining consciousness.
Methods:
1. Sixty clean level Wistar rats were randomized into five groups including blank group, model group, AAR group and control group. After successfully modeling, the AAR group was treated by needling Neiguan, Renzhong, Yintang, Baihui caves for four weeks. The control group was treated by needling Shenshu, Zusanli caves. The other two groups were given no treatment.
2. To observe the behavior change of the rats through Morris water maze test the day after the last treatment.
3. To observe the neuronal apoptosis in the hippocampus tissue of Alzheimer's rats by Tunel assay after the last day of Morris water maze test.
4. To observe the expression of Bcl-2, Bax and Caspase-3 in the hippocampus tissue of Alzheimer's rats by immu-nohistochemical method.
Results:
1. From the second day of orientation navigation experiments, the escaping latency was significantly shorter in the AAR group and the control group.The downward trend was more obvious in the AAR group, with significant differences compared with the control group (P < 0.05). In the observation of percentage of the second quadrant time and traversing times, it showed marked increasing in the AAR group and the control group.But a higher trend was showed in the AAR group compared with the control (P < 0.05).
2. The number of apoptosis cells in the AAR group and the control group was obviously fewer than that in the model group.
3. The expression of Bcl-2, compared with the model group, was increased in the AAR group and the control
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