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4. Discussion The present study demonstrated that acupuncture DU20 through GB7 could regulated the brain content of TNF-a and NFkB induced by ICH in a rat model. TNF-a activates NFkB, and by doing so, reduces the integrity of the blood brain barrier, aggravates the development of cerebral edema. Inhibiting the expression of TNF-a/ NFkB could reduce inflammatory responses, reduce nerve injury, and improve the recovery of neurological func-tion.4 Examination of the protein content of TNF-a and NFkB in brain tissue in the current study showed reduced expression of TNF-a and NFkB by SA, suggesting that SA could inhibit inflammatory reaction. We speculate that SA produces its neuroprotective action against cerebral hemorrhage by influencing multiple molecular targets.
5. Conclusion SA could regulates TNF-a and NFkB expression in a rat model of hemorrhagic stroke. References
1. Uchida S, Hotta H. Acupuncture affects regional blood flow in various organs. Evid Based Complement Altern Med. 2008;5:145-151.
2. Yang A, et al. Acupuncture for stroke rehabilitation. Cochrane Database Syst Rev. 2016;26:CD004131.
3. Liu, et al. Scalp acupuncture attenuates neurological deficits in a rat model of hemorrhagic stroke. Complement Ther Med. 2017;32:85-90.
4. King MD, et al. Attenuation of hematoma size and neurological injury with curcumin following intracerebral hemorrhage in mice. J Neurosurg. 2011; 115:116-123.
COMPARATIVE OBSERVATION OF EARLY STAGE SCAPULOH-UMERAL PERIARTHRITIS TREATED WITH KINETIC ACUPUNCTURE ON DISTAL POINTS OFTHE AFFECTED MERIDIANS
XiaKunpeng
The SecondAffiliated Hospital of Heilongjiang TCM University, 150001, Harbin , China ; Email : xkp850713@126.
com
Abstarct To compare the clinical therapeutic effects on scapulohumeral periarthritis between kinetic acupuncture on distal points of the affected meridians and shoulder three-needle therapy.
Keywords:Kinetic Acupuncture on the Distal Points of the Affected ; Meridians;Scapulohumeral Periarthritis;Shoul-der Three-Needle Therapy
Objective:To compare the clinical therapeutic effects on scapulohumeral periarthritis between kinetic acupuncture on distal points of the affected meridians and shoulder three-needle therapy.
Methods : Fifty cases of scapulohumeral periarthriris from the acupuncture clinic of the second affiliated hospital of Heilongjiang University of Chinese Medicine were randomly divided into an observation group and a control group, 25 cases for each. The cases in observation group were treated with kinetic acupuncture on the distal points of the affected meridians. For example, the case of Hand-Taiyin type was treated with Yúji(LU 10) and the case of Hand-Yangming type was treated with Hégú(LI4),etc. The cases in control group were treated with shoulder three-needle therapy, in which, Jiánqián(Extral), Jiányú(LI5),Jiánliáo(TE14) were selected; 7 days of treatment made one session, totally 2 sessions were required. Results before and after treatment was evaluated with Short Form McGill Pain Questionnaire score(SF-MPQ). Statistics and analysis was done using SPSS statistical software.
Results : 1. Comparison the two groups shoulder joint efficacy scores. In observation group, the curative rate based
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on shoulder joint was 72.0% (18/25), which was superior to that of 40.0% (10/25) in control group.The observation group showed tendency to be more effective than the control group.2. Comparing the two groups subjective feeling of pain rating index (PRI-A) showed statistically significant decreasing intervention curve among the two groups (F=127.196 P<0.001). The observation group expressed after treatment a larger decline than the control group.3. Comparing the total score of pain sensation according to emotional items (PRI-B) between the two groups. Results showed that there was a statistical difference (F=9.776P=0.003) between the two groups, comparing the decline of their respective curve on this issue. The observation group decline more than the control group.4. Comparison between the two groups of the total assessed pain rating index score (PRI, subjective pain feeling item + emotion item). Results after comparing the two groups, showed that there was a statistical difference (F=116.812P<0.001) between the two groups, comparing the decline of their respective curves on this issue. The observation group decline more than the control group.5. Comparison between the two groups according to the rated Visual Analog Scale (VAS) assessed data. Results showed that there was a statistical difference (F=416.263P<0.001) between the two groups, comparing the decline of their respective curve on this issue. The observation group decline more than the control group.6. A comparison of the"Present Pain Index (PPI) between the two groups. Results showed that in the two groups, the decreased curve of PPI-score after the intervention expressed statistical significance(F=5.119P=0.028). The observation group showed a better decline curve than the control group.
Conclusion : From the results of the clinical and experimental researeh, we consider that kinetic acupuncture on distal points of the affected meridians improve the symptom of scapulohumeral periarthritis, but the kinetic acupuncture on distal points of the affected meridians is superior than the shoulder three-needle therapy group. The treatment that giving kinetic acupuncture on distal points of the affected meridians can relieve the pain, improve the function of the shoulder. It has the advantage of low complications, economic and convenience, so the treatment is easily accepted by the patients.
References:
[1] Melzack R. The McGill Pain Questionnaire: major properties and scoring methods[J].Pain,1975, 1(3):277—299.
[2] .KleinhenzJ.AmJAeupunet, 1991;19(3):247—253.
[3] ..KitZinRerE.AkuunkturTheorieundPraxis, 1991;19(4):220—228.
EFFECTS OF TIMOSAPONINS ON DIABETIC ENCEPHALOPATHYRATS INDUCED BY STREPTOZOTOCIN
XiaLei *, HaiyanLiu g, XiaolinSu,Yuan Li ,
Hei Long Jiang University of Traditional Chinese Medicine,No.39 Guang Hua Street Qiang Jin District Jia Mu Si 154100
P.R.China
E-mail address: [email protected](Xia Lei); [email protected](Haiyang Liu); [email protected](Xiaolin Su); [email protected](Yuan Li)*Corresponding Author. Tel.:+8618045454111; Fax:0454-8782701
BACKGROUND:Many synthesized drugs used for diabetic encephalopathy (DE) treatment with clinical severe side effects. Therefore, it is urgent and necessary to identify natural and safe agents to remedy DE. Total timosaponin(TT) isone kindof major constituent in Anemarrhena asphodeloides Bunge which is widely used in traditional Chinese medicine[1, 2]. Timosaponinexhibits various activities, including anti-inflam-matory[3, 4], hypoglycemic[5]and therapeutic effect on cognitive and behavioral impairment[6], calcium mobilization in vascular endothelial and smooth muscle cells[7], anticancer[8, 9],antiplatelet and antithrombot-ic[10]. However, the anti-DE effects and potential mechanism(s) of TT have not been previously reported.
OBJECTIVE:To observe the changes of hippocampal NF-kB pathway in streptozotocin (STZ)-induced diabetic mice treated with TT, and explore the relationship between hypoglycemic and therapeutic effect of behavioral impairment, and weather has relationship with anti-oxidative and anti-apoptosis. To investigate the treatment mechanism of TT against DE is to provide experimental basis for treating complication of diabetes in clinical.
METHODS: TT was isolated from A. asphodeloides Bunge using macroporous adsorption resin and preparative high-performance liquid chromatography. Balb/C mice was intraperitoneal injection with STZ 60, 80, 100mg-kg-1in three consecutive days respectively. The mice which was selected fasting blood glucose higher 16.67mmol-L-1received TT 100mg-kg-1 by gavage after 35 days The effect of TT on behavior changes was evaluated using morris water maze 90 days later. Blood glucose level was measured by rapid blood sugar device, colorimetric methodwas used to measure malondialdehyde (MDA) and dehydrogenase (LDH), ultraviolet spectroscopywas used to measure superoxide dismutase (SOD) and Griess to measure nitric oxide (NO)level. An ELISA assay kit was used to measure inflammatory cytokines interleukin-6(IL-6) and tumor necrosis factor-alpha(TNF-a) and hematoxylin and eosin(HE)dye to observe brain histopathological change. The expression of Bax, Bcl-2and nuclear transcription factor-kappaB (NF-kB) protein was also measured using Western blot analysis.
RESULTS:Morris water maze behavioral test showing TT protective effects on learning and memory abilities injury. Compared to model group,TT reduced the escape latency time in the training trial and increased the swimming time in the target quadrant in the probe trial. TT significantly decreased the blood glucose levels and ameliorated hippocampus histopathological injury byHE method. TT treatment notably decreasedMDA which are key biomarkers of brain oxidative stress and attenuated the reduction ofLDH. lactate levels, and enhancedSODavtivity and NO level.
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