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Материалы конференции / Conference proceedings
Материалы конференции / inference proceedings
6-ой Международный семинар «Цифровые управляемые лекарства на стыке наук» / 6th Joint Conference with International Participation «Digital medicines at the intersection of sciences»
© BEREZOVSKI M. V., VEPRINTSEV D. V. UDC 576.311.31
DOI: 10.20333/25000136-2022-5-105
Breast cancer-derived extracellular vesicles are the source of functional metabolic enzymes as potential targets for cancer therapy
M. V. Berezovski1, D. V. Veprintsev2
'University of Ottawa, Ottawa ON K1N 6N5, Canada
laboratory for Digital Controlled Drugs and Theranostics, Federal Research Center «Krasnoyarsk Science Center SB RAS», Krasnoyarsk 660036, Russian Federation
Abstract. In this work, intracellular vesicles (MVs) of breast cancer were studied. These vesicles are a source of potential targets for breast cancer therapy. This study shows that MVs carry functional metabolic enzymes and provides a framework for future studies of their biological role in BC and potential in therapeutic applications.
Key words: microvesicles, metastatic breast cancer, ornithine aminotransferase, transaldolase, bleomycin hydrolase.
Conflict of interest. The authors declare the absence of obvious and potential conflicts of interest associated with the publication of this article.
Citation: Berezovski MV, Veprintsev DV. Breast cancer-derived extracellular vesicles are the source of functional metabolic enzymes as potential targets for
cancer therapy. Siberian Medical Review. 2022;(5):105. DOI: 10.20333/25000136-2022-5-105
КРАСНОЯРСКИЙ МЕДИЦИНСКИЙ УНИВЕРСИТЕТ
1942/2022
Membrane-derived extracellular vesicles, referred to as microvesicles (MVs), have been proposed to participate in several cancer diseases [1]. In this study, MV fractions were isolated by differential ultracentrifugation from a metastatic breast cancer (BC) cell line MDA-MB-231 and a non-cancerous breast cell line MCF10A, then analysed through nano-liquid chromatography coupled to tandem mass spectrometry. A total of 1519 MV proteins were identified from both cell lines. The data obtained were compared to previously analysed proteins from small extracellular vesicles (sEVs), revealing 1272 proteins present in both MVs and sEVs derived from the MDA-MB-231 cell line. Among the 89 proteins unique to MDA-MB-231 MVs, three enzymes: ornithine aminotransferase (OAT), transaldolase (TALDO1) and bleomycin hydrolase (BLMH) were previously proposed as cancer therapy targets. These proteins were enzymatically validated in cells, sEVs, and MVs derived from both cell lines. The specific activity of OAT and TALDO1 was significantly higher in MDA-MB-231-derived MVs than in MCF10A MVs. BLMH was highly expressed in MDA-MB-231-derived MVs compared to MCF10A MVs. This study shows that
MVs carry functional metabolic enzymes and provides a framework for future studies of their biological role in BC and potential in therapeutic applications.
This research was funded by the Ministry of Science and Higher Education of the Russian Federation project FWES-2022-0005.
References
1. Pedersen S, Jensen KP, Honoré B, Kristensen SR, Pedersen CH, Szejniuk WM, Maltesen RG, Falkmer U. Circulating microvesicles and exosomes in small cell lung cancer by quantitative proteomics. Clinic Proteomics. 2022;19(1):2. DOI: 10.1186/s12014-021-09339-5
Author information
Maxim V Berezovski, Professor, PhD, Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada; 2John L. Holmes Mass Spectrometry Facility, Faculty of Science, University of Ottawa, Ottawa, ON K1N 6N5, Canada;e-mail: [email protected]
Dmitry V. Veprintsev, Cand. Phis.-Math. Sci., Senior Researcher, Krasnoyarsk Scientific Center of the SB RAS; Address: 50, Akademgorodok, Krasnoyarsk, Russian Federation 660036; Phone:+7(391)2907988; e-mail [email protected]
Received 17 June 2022 Revision Received 21 August 2022 Accepted 30 August 2022
Сибирское медицинское обозрение. 2022;(5):105
105
