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HayKOBHH BicHHK .HbBiBCbKoro Ha^0Ha№H0ro ymBepcurery BeTepHHapHOi MegnuUHH Ta 6i0TexH0H0riH iMeHi C.3. f^H^Koro Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z. Gzhytskyj
doi: 10.15421/nvlvet6623
ISSN 2413-5550 print ISSN 2518-1327 online
http://nvlvet.com.ua/
UDC 619: 616. 981. 49: 619, 612. 017, 636. 2
Antioxidant protection and peroxydation lipid of body cows of high calves for the prevention of salmonella under the influence of nanpreparation Germakap
P.Y. Lavriv [email protected]
Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z. Gzhytskyi,
Pekarska Str., 50, Lviv, 79010, Ukraine
The article deals with the results of experimental research data of relationship between the antioxidant defense system and lipid body peroxidation of high calves cows under the influence of nanpreparation Germakap conducted with simultaneous vaccination with inactivatedformol vaccination with repeated in two weeks later at the same doses as their stability and immunity to Salmonella. It was found the likely increase in activity of glutathione peroxidase and glutathione reductase and at the same time, reduction of malon dialdehyde and hydroperoxides lipids and superoxidimutase. These changes in animals body occur due to complex components adaptive nan preparation Germakap that lead to the normalization of metabolic and free radical processes in animals. However, the increase in the catalytic activity of glutathione peroxidase and glutathione reductase activity in plasma of high calves cows from research group can be explained by increasing intensity of synthesis in which these enzymes by introducing nan preparation Germakap closely associated with the regeneration of glutathione in the cell, and also the activity of glutathione peroxidase. Through interaction with restoration of glutathione and glutathione peroxidase, glutathione reductase it was formed glutathione system that protects cells from stress peroxidation
Key words: cows, antioxidant system, lipid peroxidation (lipid peroxidation), malonic dialdehyde, superoxide dismutase, glutathione peroxidase, glutathione reductase, salmonellosis, nan preparation Germakap.
Антиоксидантний захист та перокисне окиснення лшвдв оргашзму високотшьних кор1в при профшактищ сальмонельозу за впливу нанопрепарату Гермакапу
П.Ю. Лавр1в [email protected]
Львiвський нацюнальнийутверситет ветеринарноймедицини та бютехнологт iMeHi С.З. Гжицького,
вул. Пекарська, 50, м. Львiв, 79010, Украна
У статтi приведено дат результату експериментального до^дження взаeмозалежностi мiж антиоксидантною системою захисту та перокисним окисненням лiпiдiв оргашзму високотшьних корiв за впливу нанопрепарату Гермакапу з одночасною проведеною вакцинащею тактивованою формолгалуневою вакциною iз повторною ревакцинащею через два тижю у тт самш дозi та станом стiйкостi iмунiтету у них до сальмонельозу. При цьому виявлено вiрогiдне тдвищення активностi глутатiонпероксидази i глутатюнредуктази, а заодно, зниження вмiсту малонового дiальдегiду i гiдропереки-Ыв лiпiдiв та супероксидисмутази. Даш змти в органiзмi тварин вiдбуваються завдяки комплексны адаптивтй дп склад-ниюв нанопрепарату Гермакапу, яш призводять до нормалiзацii метаболiчних та выьнорадикальних процеЫв в органiзмi тварин. Однак, зростання каталтично! активностi глутатiонпероксидази та активностi глутатюнредуктази в плазмi кровi високотшьних корiв до^дно! групи можна пояснити зростанням iнтенсивностi синтезу в них даних ферментiв завдяки введенню нанопрепарату Гермакапу, що ткно пов'язано з регенеращею глутатюну в клтит, а також самою
Citation:
Lavriv, P.Y. (2016). Antioxidant protection and peroxydation lipid of body cows of high calves for the prevention of salmonella under the influence of nanpreparation Germakap. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 2(66), 112-115.
активтстю глутатюнпероксидази. Через взаемоЫю вiдновленного глутатiону з глутатiонпероксидазою i глутатiонреду-ктазою утворюеться глутатiонова система, що захищае клтини вiд пероксидного стресу
Ключовi слова: корови, антиоксидантна система, ПОЛ (перокисне окиснення лiпiдiв), малоновий дiальдегiд, суперок-сиддисмутаза, глутатiонпероксидаза, глутатюнредуктаза, сальмонельоз, нанопрепарат Гермакап.
Антиоксидантная защита и перокисное окисления липидов организма высокостельных коров при профилактике сальмонеллеза за влияния нанопрепарата Гермакапа
П.Ю. Лаврив [email protected]
Львовский национальный университет ветеринарной медицины и биотехнологий имени С.З. Гжицкого,
ул. Пекарская, 50, г. Львов, 79010, Украина
В статье приведены данные результата экспериментального исследования взаимозависимости между антиоксидант-ной системой защиты и перокисным окиснениям липидов организма высокостельных коров за влияния нанопрепарату Гермакапу с одновременной проведенной вакцинацией инактивированной формолгалуневой вакциной с повторной ревакцинацией через две недели в той же дозе и состоянием устойчивости иммунитета у них к сальмонеллеза.
При этом выявлено достоверное повышение активности глутатионпероксидазы и глутатионредуктазы, а заодно, снижения содержания малонового диальдегида и гидроперекисей липидов и супероксидисмутази. Данные изменения в организме животных происходят благодаря комплексному адаптивному действию составляющих нанопрепарату Гермакапу, которые приводят к нормализации метаболических и свободнорадикальных процессов в организме животных. Однако, рост каталитической активности глутатионпероксидазы и активности глутатионредуктазы в плазме крови высокостельных коров опытной группы можно объяснить ростом интенсивности синтеза в них данных ферментов благодаря введению нанопрепарату Гермакапу, что тесно свъязано с регенерацией глутатиона в клетке, а также самой активностью глутатионпероксидазы. Через взаимодействие востановленого глутатиона с глутатионпероксидазой и глутатионредук-тазой образуется глутатионовая система, что защищает клетки от пероксидного стресса
Ключевые слова: коровы, антиоксидантная система, ПОЛ (перокисне окисления липидов), малоновый диальдегид, су-пероксиддисмутаза, глутатионпероксидаза, глутатионредуктаза, сальмонеллез, нанопрепарат Гермакап.
Introduction
Currently, the agricultural sector of Ukraine is the issue of increasing the production of animal products, namely milk and meat from improving their quality and safety. The fact should not be forgotten that infectious diseases of farm animals and reduce the productivity of the industry significantly increase production costs. However, an important role in preventing the emergence and development of inflammation in infectious diseases plays a strengthening process of free radical oxidation. At the same time opposed to this process exists antioxidant protection, namely the system of antioxidant enzymes and natural antioxidants (Lavriv, 2011; Tiutiun, 2011; Gutyj, 2013; Gutyj, 2015; Avdos'yeva et al., 2015; Martyshuk et al., 2016).
Because the question of finding new and effective ways to prevent salmonellosis is the most important in modern veterinary medicine. Development and implementation nan preparation Germakap that based on modern nanotechnology with cheap, affordable source of micronutrients, based nano carboxylates germanium, zinc and silver are priorities as they do not cause the adaptation by pathogenic microorganisms not accumulate in tissues and organs, do not give side effects and are not harmful to the animals and the environment (Lebr and Mazerol, 2009; Avdos'yeva et al., 2015).
The purpose and objectives of the study. The aim of this work was to study the role of the new nano preparation of Germakap in the prevention of salmonellosis of
highcalves cows and increase antioxidant protection and lipid peroxidation in their body for his actions.
The purpose of the work was to study the influence no preparation Germakap together with vaccination against salmonella in antioxidant protection and lipid peroxida-tion in their body and on the growth and survival of calves in economic conditions.
Material and methods
Research for the study no preparation Germakap conducted in terms of agricultural production cooperative Mykhailo Grushevskyj (St. Number 20536228), Rohatyn district, Ivano-Frankivsk region for cattle, black and white breed, age 3 - 5 years. After a clinical examination was formed two groups of cows over 10 goals each, two months before the birth. Cow as the first control, so the second experimental group for 50 - 60 days for delivery was administered intramuscularly at a dose of 10,0 cm3 and repeated after 8 - 10 days at a dose of 15 cm3 for-molgalunic inactivated vaccine, and in addition to cows while the second group was injected y the muscle nano-preparation Germakap at a dose of 5,0 cm3 down at intervals of 8 - 10 days.
The material of the study was the blood that were taken from cows undernail artery at baseline 2 days after the formation of groups 10 days after intramuscular nano preparation and vaccination and 30 days after the first injection,
Blood plasma was determined according to the procedures:
- The number of red blood cells - by counting chamber Goryaeva;
- The concentration of hemoglobin - the method of Sali;
- Morpho-functional and antioxidant body condition of cows and calves before and after injection of these drugs is determination of hydroperoxides lipids, malondialdehyde (MDA), superoxide dismutase activity and the activity of glutathione and the activity of glutathi-one reductase assessed by morphological and specific antioxidant and immunological parameters of blood, which determined in accordance with conventional methods (Vlizlo et al., 2012).
The results were treated statistically using standard computer programs. The difference between the two values considered probable by: P < 005; P < 001; P < 0001.
Results and discussion
Analysis of the results of our studies show that the use of the time and for the period Germakap study overall clinical condition high calves cows and calves in both groups after both the control and experimental remained satisfactory and no different adverse reactions manifested. However, calves research groups use nanpreparation Germakap led to a significant improvement in appetite, coat became shiny, significantly increased their activity and increases reach to 756 - 845 grams of live weight per day. At the same time, analysis of laboratory tests of blood from cows Below Table 1 indicates that the experimental group of cows on the 30th day after administration is nanpreparation Germakap reduction of red blood cells from the top to the bottom of the physiological norm at 7.02%, and therefore to the control group to 5.36%. It was also notes among research groups at the end of the
Biochemical parameters of blood vysokotilnyh cows
experiment, hemoglobin was decreased by 2.54%, as compared to the control decline was 1.71%.
At the same time, the analysis of the study showed that the concentration of lipid hydroperoxides on the 30th day of the experiment, the experimental group significantly decreased by 35.63% (P < 0001), as compared to 32.62% in the control (P < 0001). It is noted also possible decline at the end of the experiment, the experimental group malondialdehyde concentration to 39.59% (P < 0001) and relative control group it is 37.48% (P < 0001). If we analyze the activity of superoxide dis-mutase it is necessary to note the possible decline of the research group at the end of the experiment to 20.13% (P < 0001) and in accordance with the control group is also likely to decrease 18.54% (P < 0001).
On one side are not changes in the activity of glutathi-one peroxidase and glutathione reductase. Thus, among the experimental group at the end of the experiment is likely to increase the activity of glutathione peroxidase 37.07% (P < 0001) and relative control group significant increase of 32.49% (P < 0001). At the same time, at the end of the experiment is significant increase glutathione reductase activity among the experimental group 1.9 times (P < 0001) and in accordance with the control group 1.7 times (P < 0001). It was also notes the significant increase of serum complementary activities among research groups at the end of the experiment and in accordance with the control group 1.7 times (P < 0001).
Thus, our results confirm that the introduction of this drug of high calves cows in them is likely lowering lipid hydroperoxides and malondialdehyde and significant increase in plasma activity of glutathione peroxidase and glutathione reductase, which confirms its impact on the activity of antioxidant system in their body.
Table 1
Tainian black and white dairy breed (M ± m), n = 10
Indicators Group of cows
Research Control
End Start experiment End of experience Beginning of experience End of experience
Red blood cells, 12 / L 5.7 ± 0.5 5.3 ± 0.3 5.7 ± 0.4 5.6 ± 0.5
Haemoglobin, gramm/l 106.4 ± 5.1 103.7 ± 3.4 106.3 ± 5.8 105.5 ± 4.3
The concentration of hydroperoxides lipid od.E 480 / ml odes.E 480/mji 5.36 ± 0.05 3.45 ± 0.03*** 5.35 ± 0.06 5.12 ± 0.04
The concentration of malonic dialdehyde, nmol / ml 6.82 ± 0.07 4.12 ± 0.04*** 6.76 ± 0.07 6.59 ± 0.06
Act. Superoxidedysmutazy, um.od / mg protein 1.54 ± 0.8 1.23 ± 0.5*** 1.55 ± 0.8 1.51 ± 0.7
Act. Glutathione peroxidase, mmol GSH / min. mg protein 21.42 ± 0.41 29.36 ± 0.49*** 21.45 ± 0.42 22.16 ± 0.43
Akt.hlutationredukpots, nmol NADH + / min • mg protein squirrel 0.39 ± 0.07 0.75 ± 0.09*** 0.38 ± 0.07 0.44 ± 0.08
Complementive act. Cheese, blood from 0.03±0.003 0.05±0.004 0.03±0.003 0.03±0.003
Note: *-P < 005; **- R < 001; ***- R < 0001.
However, it should be noted that the active protection of cows involving natural resistance mechanisms are often not effective due to the spread of metabolic abnor-
malities, among which an important place is a violation of the imbalance of free radical processes including lipid peroxidation and reduced antioxidant systems. However,
free-radical processes (GRP) - a necessary element of any inflammation associated with phagocytes production of reactive oxygen species (ROS).
Produced secretory function of phagocytes in the evolution needed to kill bacteria, but a sharp increase in oxygen consumption during phagocytosis sometimes results in changing the restoration of O2 to H2O in leukocytes, which mainly generate ROS. However, excess ROS initiates free radical lipid peroxidation (LPO) that damage tissues primarily biological membranes. The emergence of a large number of free radicals penalized in the mitochondrial electron transport chain; separation of oxidative phosphorylation under POL leads to a deep deficit of energy; changing the function of enzymes, carbohydrates and proteins, including proteins DNA and RNA. As a result of this, cell lost regulatory functions, abnormal proteins are appeared and are stimulated, besides the direct damaging actions, secondary destructive processes. Originating deep breach the membrane and the next - a total architectonics cell leads to its death that oxidative stress. It was also noted that a significant role in the ethi-opathogenesis play salmonella bacterial flora, which is closely linked to the activity of free radicals and immune responses, GRP is one of the parts of the course and salmonella.
Conclusions
The analysis of data obtained by us in the study, suggest stimulating effect of nanpreparation Germakap not only on the activity of the immune system in the body of high calves cows, but also on the activity of antioxidant system, which explains its effect on the function of anti-oxidant system, aimed at the disposal of toxic products of free radical oxidation and maintain bioradical physiological balance in the prevention of salmonellosis (Lebr and Mazerol, 2009; Tiutiun, 2011; Avdos'yeva et al., 2015).
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Cmammn nadiumm do peda^iï 13.09.2016
